Directional Topography Influences Adipose Mesenchymal Stromal Cell Plasticity: Prospects for Tissue Engineering and Fibrosis.

Autor: Liguori GR; University of Groningen, University Medical Center Groningen, Department of Pathology and Medical Biology, Groningen, Netherlands.; Laboratório de Cirurgia Cardiovascular e Fisiopatologia da Circulação (LIM-11), Instituto do Coração (InCor), Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, Brazil., Zhou Q; University of Groningen, University Medical Center Groningen, Department of Biomedical Engineering-FB40, W.J. Kolff Institute for Biomedical Engineering and Materials Science-FB41, A. Deusinglaan 1, 9713 AV, Groningen, Netherlands.; Institute for Translational Medicine, State Key Laboratory of Bio-fibers and Eco-textiles, Qingdao University, Qingdao 266021, China., Liguori TTA; University of Groningen, University Medical Center Groningen, Department of Pathology and Medical Biology, Groningen, Netherlands.; Laboratório de Cirurgia Cardiovascular e Fisiopatologia da Circulação (LIM-11), Instituto do Coração (InCor), Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, Brazil., Barros GG; University of Groningen, University Medical Center Groningen, Department of Pathology and Medical Biology, Groningen, Netherlands.; Laboratório de Cirurgia Cardiovascular e Fisiopatologia da Circulação (LIM-11), Instituto do Coração (InCor), Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, Brazil., Kühn PT; University of Groningen, University Medical Center Groningen, Department of Biomedical Engineering-FB40, W.J. Kolff Institute for Biomedical Engineering and Materials Science-FB41, A. Deusinglaan 1, 9713 AV, Groningen, Netherlands., Moreira LFP; Laboratório de Cirurgia Cardiovascular e Fisiopatologia da Circulação (LIM-11), Instituto do Coração (InCor), Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, Brazil., van Rijn P; University of Groningen, University Medical Center Groningen, Department of Biomedical Engineering-FB40, W.J. Kolff Institute for Biomedical Engineering and Materials Science-FB41, A. Deusinglaan 1, 9713 AV, Groningen, Netherlands., Harmsen MC; University of Groningen, University Medical Center Groningen, Department of Pathology and Medical Biology, Groningen, Netherlands.
Jazyk: angličtina
Zdroj: Stem cells international [Stem Cells Int] 2019 May 05; Vol. 2019, pp. 5387850. Date of Electronic Publication: 2019 May 05 (Print Publication: 2019).
DOI: 10.1155/2019/5387850
Abstrakt: Introduction: Progenitor cells cultured on biomaterials with optimal physical-topographical properties respond with alignment and differentiation. Stromal cells from connective tissue can adversely differentiate to profibrotic myofibroblasts or favorably to smooth muscle cells (SMC). We hypothesized that myogenic differentiation of adipose tissue-derived stromal cells (ASC) depends on gradient directional topographic features.
Methods: Polydimethylsiloxane (PDMS) samples with nanometer and micrometer directional topography gradients (wavelength ( w ) = 464-10, 990 nm; amplitude ( a ) = 49-3, 425 nm) were fabricated. ASC were cultured on patterned PDMS and stimulated with TGF- β 1 to induce myogenic differentiation. Cellular alignment and adhesion were assessed by immunofluorescence microscopy after 24 h. After seven days, myogenic differentiation was examined by immunofluorescence microscopy, gene expression, and immunoblotting.
Results: Cell alignment occurred on topographies larger than w = 1758 nm/ a = 630 nm. The number and total area of focal adhesions per cell were reduced on topographies from w = 562 nm/ a = 96 nm to w = 3919 nm/ a = 1430 nm. Focal adhesion alignment was increased on topographies larger than w = 731 nm/ a = 146 nm. Less myogenic differentiation of ASC occurred on topographies smaller than w = 784 nm/ a = 209 nm.
Conclusion: ASC adherence, alignment, and differentiation are directed by topographical cues. Our evidence highlights a minimal topographic environment required to facilitate the development of aligned and differentiated cell layers from ASC. These data suggest that nanotopography may be a novel tool for inhibiting fibrosis.
Databáze: MEDLINE
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