Cross-Reactivity Using Chimeric Trypanosoma cruzi Antigens: Diagnostic Performance in Settings Where Chagas Disease and American Cutaneous or Visceral Leishmaniasis Are Coendemic.

Autor:	Daltro RT; Gonçalo Moniz Institute, Oswaldo Cruz Foundation, Salvador, Bahia, Brazil., Leony LM; Gonçalo Moniz Institute, Oswaldo Cruz Foundation, Salvador, Bahia, Brazil., Freitas NEM; Gonçalo Moniz Institute, Oswaldo Cruz Foundation, Salvador, Bahia, Brazil., Silva ÂAO; Gonçalo Moniz Institute, Oswaldo Cruz Foundation, Salvador, Bahia, Brazil., Santos EF; Gonçalo Moniz Institute, Oswaldo Cruz Foundation, Salvador, Bahia, Brazil., Del-Rei RP; Faculty of Technology and Sciences of Bahia, Salvador, Bahia, Brazil., Brito MEF; Aggeu Magalhães Institute, Oswaldo Cruz Foundation, Recife, Pernambuco, Brazil., Brandão-Filho SP; Aggeu Magalhães Institute, Oswaldo Cruz Foundation, Recife, Pernambuco, Brazil., Gomes YM; Aggeu Magalhães Institute, Oswaldo Cruz Foundation, Recife, Pernambuco, Brazil., Silva MS; Federal University of Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil.; Institute of Hygiene and Tropical Medicine, Lisbon, Portugal., Donato ST; Federal University of Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil., Jeronimo SMB; Federal University of Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil., Monteiro GRG; Federal University of Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil., Carvalho LP; Gonçalo Moniz Institute, Oswaldo Cruz Foundation, Salvador, Bahia, Brazil.; Federal University of Bahia, Salvador, Bahia, Brazil., Magalhães AS; Federal University of Bahia, Salvador, Bahia, Brazil., Zanchin NIT; Carlos Chagas Institute, Oswaldo Cruz Foundation, Curitiba, Paraná, Brazil., Celedon PAF; Molecular Biology Institute of Paraná, Curitiba, Paraná, Brazil., Santos FLN; Gonçalo Moniz Institute, Oswaldo Cruz Foundation, Salvador, Bahia, Brazil fred.santos@bahia.fiocruz.br.
Jazyk:	angličtina
Zdroj:	Journal of clinical microbiology [J Clin Microbiol] 2019 Jul 26; Vol. 57 (8). Date of Electronic Publication: 2019 Jul 26 (Print Publication: 2019).
DOI:	10.1128/JCM.00762-19
Abstrakt:	Chimeric T. cruzi antigens have been proposed as a diagnostic tool for chronic Chagas disease (CD) in both settings where Chagas disease is endemic and those where it is not endemic. Antibody response varies in accordance to each T. cruzi strain, presenting challenges to the use of antigens lacking demonstrated cross-reactivity with Leishmania spp. Our group expressed four chimeric proteins (IBMP-8.1, IBMP-8.2, IBMP-8.3, and IBMP-8.4) and previously assessed their diagnostic performance to determine cross-reactivity with Leishmania spp. Here, we validated our findings using serum samples from different Brazilian geographic areas reporting endemic Chagas disease, endemic visceral or American cutaneous leishmaniasis (ACL), or both. Overall, 829 serum samples were evaluated using commercial and IBMP enzyme-linked immunosorbent assays. Due to the absence of a reference assay to diagnosis CD, latent class analysis (LCA) was performed through the use of a statistical model. The incidence of cross-reactivity for ACL-positive samples varied from 0.35% (IBMP-8.3) to 0.70% (IBMP-8.1 and IBMP-8.2). Regarding visceral leishmaniasis (VL)-positive samples, the IBMP-8.2 and IBMP-8.3 antigens cross-reacted with six (3.49%) and with only one sample (0.58%), respectively. No cross-reactivity with either ACL or VL was observed for the IBMP-8.4 antigen. Similarly, no cross-reactions were found when VL-positive samples were assayed with IBMP-8.1. The agreement among the results obtained using IBMP antigens ranged from 97.3% for IBMP-8.2 and 99% for IBMP-8.1 and IBMP-8.3 to 100% for IBMP-8.4, demonstrating almost perfect agreement with LCA. Accordingly, in light of the negligible cross-reactivity with both ACL and VL, we suggest the use of IBMP antigens in regions where T. cruzi and Leishmania spp. are coendemic. (Copyright © 2019 Daltro et al.)
Databáze:	MEDLINE
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