| Autor: |
Chen F, Zhao ER, Hableel G, Hu T; School of Materials Science and Engineering , Central South University , Changsha 410083 , China., Kim T; Department of Biomedical Engineering, Institute of Quantitative Health Science and Engineering , Michigan State University , 775 Woodlot Drive , East Lansing , Michigan 48824 , United States., Li J, Gonzalez-Pech NI, Cheng DJ, Lemaster JE, Xie Y, Grassian VH; Scripps Institution of Oceanography, San Diego , 9500 Gilman Drive , La Jolla , California 92093 , United States., Sen GL, Jokerst JV |
| Abstrakt: |
Stem cell therapy in heart disease is challenged by mis-injection, poor survival, and low cell retention. Here, we describe a biocompatible multifunctional silica-iron oxide nanoparticle to help solve these issues. The nanoparticles were made via an in situ growth of Fe 3 O 4 nanoparticles on both the external surfaces and pore walls of mesocellular foam silica nanoparticles. In contrast to previous work, this approach builds a magnetic moiety inside the pores of a porous silica structure. These materials serve three roles: drug delivery, magnetic manipulation, and imaging. The addition of Fe 3 O 4 to the silica nanoparticles increased their colloidal stability, T 2 -based magnetic resonance imaging contrast, and superparamagnetism. We then used the hybrid materials as a sustained release vehicle of insulin-like growth factor-a pro-survival agent that can increase cell viability. In vivo rodent studies show that labeling stem cells with this nanoparticle increased the efficacy of stem cell therapy in a ligation/reperfusion model. The nanoparticle-labeled cells increase the mean left ventricular ejection fraction by 11 and 21% and the global longitudinal strain by 24 and 34% on days 30 and 60, respectively. In summary, this multifunctional nanomedicine improves stem cell survival via the sustained release of pro-survival agents. |
