Electron paramagnetic resonance oximetry as a novel approach to monitor the effectiveness and quality of red blood cell transfusions.

Autor: Hou H; Department of Radiology, Geisel School of Medicine at Dartmouth College, Hanover, NH, United States of America., Baek JH; Laboratory of Biochemistry and Vascular Biology, Division of Blood Components and Devices, Center of Biologics Evaluation and Research (CBER), FDA, Silver Spring, MD, United States of America., Zhang H; Department of Chemistry, Dartmouth College, Hanover, NH, United States of America., Wood F; Laboratory of Biochemistry and Vascular Biology, Division of Blood Components and Devices, Center of Biologics Evaluation and Research (CBER), FDA, Silver Spring, MD, United States of America., Gao Y; Division of Viral Products, Center of Biologics Evaluation and Research (CBER), FDA, Silver Spring, MD, United States of America., Flood AB; Department of Radiology, Geisel School of Medicine at Dartmouth College, Hanover, NH, United States of America., Swartz HM; Department of Radiology, Geisel School of Medicine at Dartmouth College, Hanover, NH, United States of America., Buehler PW; Laboratory of Biochemistry and Vascular Biology, Division of Blood Components and Devices, Center of Biologics Evaluation and Research (CBER), FDA, Silver Spring, MD, United States of America.
Jazyk: angličtina
Zdroj: Blood transfusion = Trasfusione del sangue [Blood Transfus] 2019 Jul; Vol. 17 (4), pp. 296-306. Date of Electronic Publication: 2019 May 16.
DOI: 10.2450/2019.0037-19
Abstrakt: Background: The goal of red blood cell transfusion is to improve tissue oxygenation. Assessment of red blood cell quality and individualised therapeutic needs can be optimised using direct oxygen (O 2 ) measurements to guide treatment. Electron paramagnetic resonance oximetry is capable of accurate, repeatable and minimally invasive measurements of tissue pO 2 . Here we present preclinical proof-of-concept of the utility of electron paramagnetic resonance oximetry in an experimental setting of acute blood loss, transfusion, and post-transfusion monitoring.
Materials and Methods: Donor rat blood was collected, leucocyte-reduced, and stored at 4 °C in AS-3 for 1, 7 and 14 days. Red blood cell morphology, O 2 equilibrium, p50 and Hill numbers from O 2 binding and dissociation curves were evaluated in vitro. Recipient rats were bled and maintained at a mean arterial pressure of 30-40 mmHg and hind limb muscle (biceps femoris) pO 2 at 25-50% of baseline. Muscle pO 2 was monitored continuously over the course of experiments to assess the effectiveness of red blood cell preparations at different stages of blood loss and restoration.
Results: Red blood cell morphology, O 2 equilibrium and p50 values of intra-erythrocyte haemoglobin were significantly altered by refrigerated storage for both 7 and 14 days. Transfusion of red blood cells stored for 7 or 14 days demonstrated an equivalently impaired ability to restore hind limb muscle pO 2 , consistent with in vitro observations and transfusion with albumin. Red blood cells refrigerated for 1 day demonstrated normal morphology, in vitro oxygenation and in vivo restoration of tissue pO 2 .
Discussion: Electron paramagnetic resonance oximetry represents a useful approach to assessing the quality of red blood cells and subsequent transfusion effectiveness.
Databáze: MEDLINE
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