Antioxidant action of alpha lipoic acid on the testis and epididymis of diabetic rats: morphological, sperm and immunohistochemical evaluation.

Autor:	Corrêa LBNS; Departamento de Morfologia, Laboratório de Biomorfologia Celular e Extracelular, Universidade Federal Fluminense - UFF, Niterói, RJ, Brasil., da Costa CAS; Departamento de Nutrição e Dietética, Nutrição Experimental, Universidade Federal Fluminense - UFF, Niterói, RJ, Brasil., Ribas JAS; Departamento de Fisiologia e Farmacologia, Universidade Federal Fluminense - UFF, Niterói, RJ, Brasil., Boaventura GT; Departamento de Nutrição e Dietética, Nutrição Experimental, Universidade Federal Fluminense - UFF, Niterói, RJ, Brasil., Chagas MA; Departamento de Morfologia, Laboratório de Biomorfologia Celular e Extracelular, Universidade Federal Fluminense - UFF, Niterói, RJ, Brasil.
Jazyk:	angličtina
Zdroj:	International braz j urol : official journal of the Brazilian Society of Urology [Int Braz J Urol] 2019 Jul-Aug; Vol. 45 (4), pp. 815-824.
DOI:	10.1590/S1677-5538.IBJU.2018.0774
Abstrakt:	Introduction: Chronic hyperglycemia is caused by diabetes mellitus-committed genital morphophysiology, and oxidative stress is one of the main factors involved in this process. Alpha lipoic acid (ALA) can prevent metabolic and morphological changes in diabetic individuals. Objectives: In present study, we evaluated the effects of regular ALA consumption on the spermatogenesis and histoarchitecture in the male genital system of diabetic rats. Materials and Methods: Thirty-two Wistar rats were divided into groups: Control (CG); Diabetic Control (DCG), receiving commercial diet: ALA Group (ALAG) and Diabetic ALA Group (DALAG), fed diets with added ALA (300 mg/Kg bw). The diabetic groups received a single injection of streptozotocin (60 mg/kg). After sixty days of the diet, the animals were euthanized, and semen, testis and epididymis samples were collected. A histomorphometric analysis was performed to determine the epithelial height, tubular and luminal diameter, tubular and luminal area of seminiferous tubules and each epididymal region. Sertoli cells were evidenced using the antivimentin antibody and were quantified. The results were statistically analyzed by the ANOVA test. Results: At the end of the experiment, the DALAG glycemia was signifi cantly lower than DCG. The histomorphometric parameters of the seminiferous and epididymal tubules did not show improvement in the DALAG. However, there was an improvement in the DALAG in terms of the concentration, motility and percentage of spermatic pathologies, as well as in the number of Sertoli cells (p<0.001). Conclusions: The results demonstrated that supplementation with the ALA antioxidant retards testicular lesions and preserve the process of spermatogenesis in diabetes. Competing Interests: None declared. (Copyright® by the International Brazilian Journal of Urology.)
Databáze:	MEDLINE
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