Steroidomics for highlighting novel serum biomarkers of testosterone doping.

Autor:	Ponzetto F; Swiss Laboratory for Doping Analyses, University Center of Legal Medicine Geneva & Lausanne, Centre Hospitalier Universitaire Vaudois & University of Lausanne, 1066 Epalinges, Switzerland.; Center of Research & Expertise in Anti-Doping Sciences (REDs), University of Lausanne, 1015 Lausanne, Switzerland., Boccard J; School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, 1211 Geneve, Switzerland.; Swiss Center of Applied Human Toxicology (SCAHT), University of Basel, 4055 Basel, Switzerland., Nicoli R; Swiss Laboratory for Doping Analyses, University Center of Legal Medicine Geneva & Lausanne, Centre Hospitalier Universitaire Vaudois & University of Lausanne, 1066 Epalinges, Switzerland., Kuuranne T; Swiss Laboratory for Doping Analyses, University Center of Legal Medicine Geneva & Lausanne, Centre Hospitalier Universitaire Vaudois & University of Lausanne, 1066 Epalinges, Switzerland., Saugy M; Center of Research & Expertise in Anti-Doping Sciences (REDs), University of Lausanne, 1015 Lausanne, Switzerland., Rudaz S; School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, 1211 Geneve, Switzerland.; Swiss Center of Applied Human Toxicology (SCAHT), University of Basel, 4055 Basel, Switzerland.
Jazyk:	angličtina
Zdroj:	Bioanalysis [Bioanalysis] 2019 Jun; Vol. 11 (12), pp. 1171-1187. Date of Electronic Publication: 2019 Jun 10.
DOI:	10.4155/bio-2019-0079
Abstrakt:	Aim: Quantification of testosterone (T) and 5α-dihydrotestosterone serum concentrations proved to be an efficient alternative to urinary steroid profiling for the detection of T doping. In this context, additional serum markers could be discovered by exploratory untargeted steroidomics studies. Results: Endogenous steroid metabolites were monitored by ultra high-performance liquid chromatography coupled to high-resolution mass spectrometry in serum samples collected during a T administration clinical trial. A three-step workflow for accurate review of annotation was used and multifactorial data analysis allowed highlighting promising serum biomarkers. Longitudinal monitoring of selected compounds was performed to assess T abuse detection capabilities. Conclusion: Application of serum steroidomics showed high potential for biomarker discovery of T doping, suggesting longitudinal monitoring of steroid hormones in serum as a significant improvement in detection of endogenous steroids abuse.
Databáze:	MEDLINE
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