A mini outbreak of human metapneumovirus infection with severe acute respiratory symptoms in a selected group of children presented to a teaching hospital in Sri Lanka.

Autor: Noordeen F; 1Department of Microbiology, Faculty of Medicine, University of Peradeniya, Peradeniya, Sri Lanka., Pitchai FNN; 1Department of Microbiology, Faculty of Medicine, University of Peradeniya, Peradeniya, Sri Lanka., Kudagammana ST; 2Department of Paediatrics, Faculty of Medicine, University of Peradeniya, Peradeniya, Sri Lanka.; 3Teaching Hospital, Peradeniya, Sri Lanka., Rafeek RAM; 1Department of Microbiology, Faculty of Medicine, University of Peradeniya, Peradeniya, Sri Lanka.
Jazyk: angličtina
Zdroj: Virusdisease [Virusdisease] 2019 Jun; Vol. 30 (2), pp. 307-310. Date of Electronic Publication: 2019 Apr 04.
DOI: 10.1007/s13337-019-00522-9
Abstrakt: Human metapneumovirus (hMPV) of the family Paramyxoviridae is a relatively new virus causing severe acute respiratory tract infections (SARI) in children. Data on hMPV infection in Asia including Sri Lanka is limited. We aimed to detect respiratory viruses including hMPV in a selected group of children affected by a small outbreak of SARI presented to the Teaching Hospital, Peradeniya (THP), Sri Lanka in 2014. Nasopharyngeal aspirates (NPA) were obtained from 21 children with SARI and tested for hMPV, influenza A and B, parainfluenza 1, 2 and 3 (PIV 1-3), adenovirus and respiratory syncytial virus (RSV) antigens using an immunofluorescence assay (IFA). In addition, a one step RT-PCR was done for the detection of hMPV from the viral RNA extracts. Of the 21 NPA samples tested for respiratory viral antigens by IFA, two were positive for RSV (9.5%), one was positive for influenza A (4.8%) and one was positive for both adenovirus and PIV-2 (4.8%). Of the 21 NPA viral RNA extracts tested by RT-PCR, 18 (86%) were positive for hMPV, in which 2 were co-infected with RSV and influenza A virus, respectively. hMPV was the predominant cause of SARI outbreak (2014) in children presented to the THP, Sri Lanka.
Competing Interests: Conflict of interestThe authors declare that they have no conflict of interest.
Databáze: MEDLINE
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