Drosophila Ovarian Germline Stem Cell Cytocensor Projections Dynamically Receive and Attenuate BMP Signaling.

Autor:	Wilcockson SG; Faculty of Biology, Medicine and Health, University of Manchester, Manchester M13 9PT, UK., Ashe HL; Faculty of Biology, Medicine and Health, University of Manchester, Manchester M13 9PT, UK. Electronic address: hilary.ashe@manchester.ac.uk.
Jazyk:	angličtina
Zdroj:	Developmental cell [Dev Cell] 2019 Aug 05; Vol. 50 (3), pp. 296-312.e5. Date of Electronic Publication: 2019 Jun 06.
DOI:	10.1016/j.devcel.2019.05.020
Abstrakt:	In the Drosophila ovarian germline, Bone Morphogenetic Protein (BMP) signals released by niche cells promote germline stem cell (GSC) maintenance. Although BMP signaling is known to repress expression of a key differentiation factor, it remains unclear whether BMP-responsive transcription also contributes positively to GSC identity. Here, we identify the GSC transcriptome using RNA sequencing (RNA-seq), including the BMP-induced transcriptional network. Based on these data, we provide evidence that GSCs form two types of cellular projections. Genetic manipulation and live ex vivo imaging reveal that both classes of projection allow GSCs to access a reservoir of Dpp held away from the GSC-niche interface. Moreover, microtubule-rich projections, termed "cytocensors", form downstream of BMP and have additional functionality, which is to attenuate BMP signaling. In this way, cytocensors allow dynamic modulation of signal transduction to facilitate differentiation following GSC division. This ability of cytocensors to attenuate the signaling response expands the repertoire of functions associated with signaling projections. (Copyright © 2019 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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