| Autor: |
Kusmierek M; Department of Molecular Infection Biology, Helmholtz Centre for Infection Research, Braunschweig, Germany., Heroven AK; Department of Molecular Infection Biology, Helmholtz Centre for Infection Research, Braunschweig, Germany., Beckstette M; Department of Molecular Infection Biology, Helmholtz Centre for Infection Research, Braunschweig, Germany., Nuss AM; Department of Molecular Infection Biology, Helmholtz Centre for Infection Research, Braunschweig, Germany., Dersch P; Department of Molecular Infection Biology, Helmholtz Centre for Infection Research, Braunschweig, Germany. petra.dersch@uni-muenster.de.; Institute of Infectiology, University of Münster, Münster, Germany. petra.dersch@uni-muenster.de. |
| Abstrakt: |
A detailed knowledge about virulence-relevant genes, as well as where and when they are expressed during the course of an infection is required to obtain a comprehensive understanding of the complex host-pathogen interactions. The development of unbiased probe-independent RNA sequencing (RNA-seq) approaches has dramatically changed transcriptomics. It allows simultaneous monitoring of genome-wide, infection-linked transcriptional alterations of the host tissue and colonizing pathogens. Here, we provide a detailed protocol for the preparation and analysis of lymphatic tissue infected with the mainly extracellularly growing pathogen Yersinia pseudotuberculosis. This method can be used as a powerful tool for the discovery of Yersinia-induced host responses, colonization and persistence strategies of the pathogen, and underlying regulatory processes. Furthermore, we describe computational methods with which we analyzed obtained datasets. |
