A Robust and Scalable High-Throughput Compatible Assay for Screening Amyloid-β-Binding Compounds.

Autor: McClure R; Vanderbilt University Institute of Imaging Science, Vanderbilt University, Nashville, TN, USA., Redha R; Vanderbilt High-Throughput Screening Facility, Nashville, TN, USA., Vinson P; Vanderbilt High-Throughput Screening Facility, Nashville, TN, USA.; Department of Biochemistry, Vanderbilt University, Nashville, TN, USA., Pham W; Vanderbilt University Institute of Imaging Science, Vanderbilt University, Nashville, TN, USA.; Department of Radiology and Radiological Sciences, Vanderbilt University, Nashville, TN, USA.; Department of Biomedical Engineering, Vanderbilt University, Nashville, TN, USA.; Vanderbilt Brain Institute, Vanderbilt University, Nashville, TN, USA.; Vanderbilt Ingram Cancer Center, Nashville, TN, USA.; Vanderbilt Institute of Chemical Biology, Nashville, TN, USA.; Vanderbilt Institute of Nanoscale Science and Engineering, Nashville, TN, USA.
Jazyk: angličtina
Zdroj: Journal of Alzheimer's disease : JAD [J Alzheimers Dis] 2019; Vol. 70 (1), pp. 187-197.
DOI: 10.3233/JAD-190316
Abstrakt: A robust fluorescent readout assay using topologically-sensitive dyes improves the screening of novel amyloid-binding molecules. One of the key components that make this assay more realistic is the use of endogenous amyloid obtained from 5XFAD mouse brains. The assay conditions were optimized for high throughput screening operation with Z-prime values >0.6. Using a combination of library of 3,500 compounds including known drugs, natural-derived molecules and random organic molecules, 8 unique molecules were identified as potential amyloid-binding agents.
Databáze: MEDLINE