Biotransformation of progesterone by Aspergillus nidulans VKPM F-1069 (wild type).

Autor: Savinova OS; Bach Institute of Biochemistry, Federal Research Center 'Fundamentals of Biotechnology', Russian Academy of Sciences, Leninski prospect, 33, building 2, Moscow 119071, Russian Federation. Electronic address: savinova_os@rambler.ru., Solyev PN; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilova st., 32, Moscow 119991, Russian Federation. Electronic address: solyev@gmail.com., Vasina DV; Bach Institute of Biochemistry, Federal Research Center 'Fundamentals of Biotechnology', Russian Academy of Sciences, Leninski prospect, 33, building 2, Moscow 119071, Russian Federation. Electronic address: d.v.vasina@gmail.com., Tyazhelova TV; N.I. Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow 119333, Russian Federation. Electronic address: t.tyazhelova@gmail.com., Fedorova TV; Bach Institute of Biochemistry, Federal Research Center 'Fundamentals of Biotechnology', Russian Academy of Sciences, Leninski prospect, 33, building 2, Moscow 119071, Russian Federation. Electronic address: fedorova_tv@mail.ru., Savinova TS; Faculty of Chemistry, Lomonosov Moscow State University, Leninskiye gory, 1-3, Moscow 119991, Russian Federation. Electronic address: savinova@org.chem.msu.ru.
Jazyk: angličtina
Zdroj: Steroids [Steroids] 2019 Sep; Vol. 149, pp. 108421. Date of Electronic Publication: 2019 Jun 06.
DOI: 10.1016/j.steroids.2019.05.013
Abstrakt: Biotechnological transformation of steroids using enzyme systems of microorganisms is often the only possible method to modify the molecule in the industrial production of steroid drugs. Filamentous fungus Aspergillus nidulans has been little studied as a steroid-transforming microorganism. We studied the ability of the A. nidulans VKPM F-1069 strain to transform progesterone (PG) for the first time. This strain converts PG into 3 main products: 11α-hydroxy-PG, 11α-acetoxy-PG and 6β,11α-dihydroxy-PG. It has been established that in the first stage, the hydroxylation of PG occurs into C11α position, then the formed 11α-hydroxy-PG is modified into 11α-acetoxy-PG and 6β,11α-dihydroxy-PG. It was found that changes in the composition of the growth medium, aeration and the duration of the mycelium cultivation do not affect the qualitative composition of PG transformation products, but their ratios have changed. Under conditions of limited aeration, the direction of secondary modification of 11α-hydroxy-PG is shifted towards the formation of 11α-acetoxy-PG.
(Copyright © 2019 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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