Toward a Consensus on Applying Quantitative Liquid Chromatography-Tandem Mass Spectrometry Proteomics in Translational Pharmacology Research: A White Paper.

Autor: Prasad B; Department of Pharmaceutics, University of Washington, Seattle, Washington, USA., Achour B; Centre for Applied Pharmacokinetic Research, University of Manchester, Manchester, UK., Artursson P; Department of Pharmacy, Uppsala University, Uppsala, Sweden., Hop CECA; Genentech, South San Francisco, California, USA., Lai Y; Gilead Sciences, Foster City, California, USA., Smith PC; Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA., Barber J; Centre for Applied Pharmacokinetic Research, University of Manchester, Manchester, UK., Wisniewski JR; Biochemical Proteomics Group, Max Planck Institute of Biochemistry, Martinsried, Germany., Spellman D; Pharmacokinetics Pharmacodynamics & Drug Metabolism, Merck & Co., Inc., West Point, Pennsylvania, USA., Uchida Y; Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Japan., Zientek MA; Takeda California, San Diego, California, USA., Unadkat JD; Department of Pharmaceutics, University of Washington, Seattle, Washington, USA., Rostami-Hodjegan A; Centre for Applied Pharmacokinetic Research, University of Manchester, Manchester, UK.; Certara (Simcyp Division), Sheffield, UK.
Jazyk: angličtina
Zdroj: Clinical pharmacology and therapeutics [Clin Pharmacol Ther] 2019 Sep; Vol. 106 (3), pp. 525-543. Date of Electronic Publication: 2019 Jul 26.
DOI: 10.1002/cpt.1537
Abstrakt: Quantitative translation of information on drug absorption, disposition, receptor engagement, and drug-drug interactions from bench to bedside requires models informed by physiological parameters that link in vitro studies to in vivo outcomes. To predict in vivo outcomes, biochemical data from experimental systems are routinely scaled using protein quantity in these systems and relevant tissues. Although several laboratories have generated useful quantitative proteomic data using state-of-the-art mass spectrometry, no harmonized guidelines exit for sample analysis and data integration to in vivo translation practices. To address this gap, a workshop was held on September 27 and 28, 2018, in Cambridge, MA, with 100 experts attending from academia, the pharmaceutical industry, and regulators. Various aspects of quantitative proteomics and its applications in translational pharmacology were debated. A summary of discussions and best practices identified by this expert panel are presented in this "White Paper" alongside unresolved issues that were outlined for future debates.
(© 2019 The Authors Clinical Pharmacology & Therapeutics © 2019 American Society for Clinical Pharmacology and Therapeutics.)
Databáze: MEDLINE
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