Effect of ultraviolet (UV-C) radiation on spores and biofilms of Alicyclobacillus spp. in industrialized orange juice.

Autor: Prado DBD; Postgraduate Program in Food Science, State University of Maringá, Paraná, Brazil., Szczerepa MMDA; Postgraduate Program in Food Science, State University of Maringá, Paraná, Brazil., Capeloto OA; Department of Physics, State University of Maringá, Paraná, Brazil., Astrath NGC; Department of Physics, State University of Maringá, Paraná, Brazil., Santos NCAD; Department of Statistics, State University of Maringá, Paraná, Brazil., Previdelli ITS; Department of Statistics, State University of Maringá, Paraná, Brazil., Nakamura CV; Department of Basic Health Sciences, State University of Maringá, Paraná, Brazil., Mikcha JMG; Department of Clinical Analyses and Biomedicine, State University of Maringá, Paraná, Brazil., Abreu Filho BA; Department of Basic Health Sciences, State University of Maringá, Paraná, Brazil. Electronic address: baafilho@uem.br.
Jazyk: angličtina
Zdroj: International journal of food microbiology [Int J Food Microbiol] 2019 Sep 16; Vol. 305, pp. 108238. Date of Electronic Publication: 2019 May 28.
DOI: 10.1016/j.ijfoodmicro.2019.108238
Abstrakt: Bacteria of the genus Alicyclobacillus pose serious quality problems for the juice processing industries that have sought effective alternatives for its control. The present study evaluated the effect of UV-C radiation on the reduction of spores and biofilm formation of Alicyclobacillus spp. on stainless steel and rubber surfaces using industrialized orange juice as a culture medium. Four reference Alicyclobacillus spp. species and different UV-C dosages were investigated. After exposed for 20 min (16.8 kJ/m 2 ) to UV-C, the spores of Alicyclobacillus acidoterrestris, Alicyclobacillus herbarius, and Alicyclobacillus cycloheptanicus decreased drastically more of 4 log CFU/mL, with counts below the detection limit of the method (<1.7 log CFU/mL), while the Alicyclobacillus acidocaldarius spores were more sensitive to UV-C, once this spore reduction was observed within 15 min (12.6 kJ/m 2 ). Morphological changes in the Alicyclobacillus acidoterrestris spores were observed by scanning electron microscopy. A reduction of biofilm formation was observed for all UV-C treatments, and the higher reductions (approximately 2 log CFU/mL) were found for the Alicyclobacillus acidocaldarius species after 30 min (26.2 kJ/m 2 ), on the stainless steel and rubber surfaces. The results suggest that UV-C can be used to reduce the biofilm formation and could be a promising alternative for controlling Alicyclobacillus spp. spores in industrialized orange juice.
(Copyright © 2019. Published by Elsevier B.V.)
Databáze: MEDLINE
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