bHLH-PAS protein RITMO1 regulates diel biological rhythms in the marine diatom Phaeodactylum tricornutum .

Autor:	Annunziata R; Laboratory of Computational and Quantitative Biology, Sorbonne Université, CNRS, Institut de Biologie Paris-Seine, F-75005 Paris, France.; Integrative Marine Ecology, Stazione Zoologica Anton Dhorn, 80121 Napoli, Italy., Ritter A; Laboratory of Computational and Quantitative Biology, Sorbonne Université, CNRS, Institut de Biologie Paris-Seine, F-75005 Paris, France., Fortunato AE; Laboratory of Computational and Quantitative Biology, Sorbonne Université, CNRS, Institut de Biologie Paris-Seine, F-75005 Paris, France., Manzotti A; Laboratory of Computational and Quantitative Biology, Sorbonne Université, CNRS, Institut de Biologie Paris-Seine, F-75005 Paris, France.; Laboratoire de Biologie du chloroplaste et perception de la lumière chez les microalgues UMR7141, CNRS, Sorbonne Université, Institut de Biologie Physico-Chimique, 75005, Paris, France., Cheminant-Navarro S; Laboratory of Computational and Quantitative Biology, Sorbonne Université, CNRS, Institut de Biologie Paris-Seine, F-75005 Paris, France.; Laboratoire de Biologie du chloroplaste et perception de la lumière chez les microalgues UMR7141, CNRS, Sorbonne Université, Institut de Biologie Physico-Chimique, 75005, Paris, France., Agier N; Laboratory of Computational and Quantitative Biology, Sorbonne Université, CNRS, Institut de Biologie Paris-Seine, F-75005 Paris, France., Huysman MJJ; Laboratory of Computational and Quantitative Biology, Sorbonne Université, CNRS, Institut de Biologie Paris-Seine, F-75005 Paris, France.; Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium.; Vlaams Instituut voor Biotechnologie Center for Plant Systems Biology, B-9052 Ghent, Belgium., Winge P; Cell Molecular Biology and Genomics Group, Department of Biology, Norwegian University of Science and Technology, 7491 Trondheim, Norway., Bones AM; Cell Molecular Biology and Genomics Group, Department of Biology, Norwegian University of Science and Technology, 7491 Trondheim, Norway., Bouget FY; Sorbonne Université, CNRS, UMR 7621, Laboratoire d'Océanographie Microbienne, Observatoire Océanologique, 66650 Banyuls-sur-Mer, France., Cosentino Lagomarsino M; Laboratory of Computational and Quantitative Biology, Sorbonne Université, CNRS, Institut de Biologie Paris-Seine, F-75005 Paris, France., Bouly JP; Laboratory of Computational and Quantitative Biology, Sorbonne Université, CNRS, Institut de Biologie Paris-Seine, F-75005 Paris, France.; Laboratoire de Biologie du chloroplaste et perception de la lumière chez les microalgues UMR7141, CNRS, Sorbonne Université, Institut de Biologie Physico-Chimique, 75005, Paris, France., Falciatore A; Laboratory of Computational and Quantitative Biology, Sorbonne Université, CNRS, Institut de Biologie Paris-Seine, F-75005 Paris, France; angela.falciatore@upmc.fr.; Laboratoire de Biologie du chloroplaste et perception de la lumière chez les microalgues UMR7141, CNRS, Sorbonne Université, Institut de Biologie Physico-Chimique, 75005, Paris, France.
Jazyk:	angličtina
Zdroj:	Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2019 Jun 25; Vol. 116 (26), pp. 13137-13142. Date of Electronic Publication: 2019 Jun 06.
DOI:	10.1073/pnas.1819660116
Abstrakt:	Periodic light-dark cycles govern the timing of basic biological processes in organisms inhabiting land as well as the sea, where life evolved. Although prominent marine phytoplanktonic organisms such as diatoms show robust diel rhythms, the mechanisms regulating these processes are still obscure. By characterizing a Phaeodactylum tricornutum bHLH-PAS nuclear protein, hereby named RITMO1, we shed light on the regulation of the daily life of diatoms. Alteration of RITMO1 expression levels and timing by ectopic overexpression results in lines with deregulated diurnal gene expression profiles compared with the wild-type cells. Reduced gene expression oscillations are also observed in these lines in continuous darkness, showing that the regulation of rhythmicity by RITMO1 is not directly dependent on light inputs. We also describe strong diurnal rhythms of cellular fluorescence in wild-type cells, which persist in continuous light conditions, indicating the existence of an endogenous circadian clock in diatoms. The altered rhythmicity observed in RITMO1 overexpression lines in continuous light supports the involvement of this protein in circadian rhythm regulation. Phylogenetic analysis reveals a wide distribution of RITMO1-like proteins in the genomes of diatoms as well as in other marine algae, which may indicate a common function in these phototrophs. This study adds elements to our understanding of diatom biology and offers perspectives to elucidate timekeeping mechanisms in marine organisms belonging to a major, but under-investigated, branch of the tree of life. (Copyright © 2019 the Author(s). Published by PNAS.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu