| Autor: |
Viet TD; Graduate School for International Development Cooperation (IDEC), Hiroshima University, Hiroshima 739-8529, Japan. viettran1609@gmail.com., Xuan TD; Graduate School for International Development Cooperation (IDEC), Hiroshima University, Hiroshima 739-8529, Japan. tdxuan@hiroshima-u.ac.jp., Van TM; Graduate School for International Development Cooperation (IDEC), Hiroshima University, Hiroshima 739-8529, Japan. truongmaivan1991@gmail.com., Andriana Y; Graduate School for International Development Cooperation (IDEC), Hiroshima University, Hiroshima 739-8529, Japan. yusufandriana@yahoo.com., Rayee R; Graduate School for International Development Cooperation (IDEC), Hiroshima University, Hiroshima 739-8529, Japan. r.rayee12@yahoo.com., Tran HD; Faculty of Biotechnology, Nguyen Tat Thanh University, 298A-300A Nguyen Tat Thanh Street, Ward 13, District 4, Ho Chi Minh City 72820, Vietnam. thdung@ntt.edu.vn. |
| Jazyk: |
angličtina |
| Zdroj: |
Medicines (Basel, Switzerland) [Medicines (Basel)] 2019 Jun 04; Vol. 6 (2). Date of Electronic Publication: 2019 Jun 04. |
| DOI: |
10.3390/medicines6020064 |
| Abstrakt: |
Background: In this study, column chromatography was applied to separate active fractions from the ethyl acetate extract of Celastrus hindsii , a medicinal plant widely used in Southern China, Northern Vietnam, Myanmar, and Malaysia. Methods: Fourteen fractions from different dilutions of chloroform and methanol were separated by column chromatography and examined for biological activities. Results: It was found that a dilution of 50-70% methanol in chloroform yielded the highest total phenolics, flavonoids, and antioxidant activities (1,1-dipheny1-2-picrylhydrazyl (DPPH), 2,2-azinobis (3-ehtylbenzothiazoline-6-sulfonic acid), diammonium salt (ABTS) radical scavenging activity, and β -carotene bleaching method measured by lipid peroxidation inhibition). In addition, by gas chromatography-mass spectrometry (GC-MS) and electrospray ionization-mass spectrometry (ESI-MS) analyses, fifteen principal compounds from bioactive fractions belonging to fatty acids, amides, flavonoids, sterols, terpenes, and phenols were identified. Of these compounds, α-amyrin, β-amyrin, hydrazine carboxamide, hexadecanoic acid, fucosterol, (3β)-D:C-friedours-7-en-3-ol, rutin, and 2-hydroxy-1-ethyl ester accounted for maximal quantities, whilst concentrations of other constituents were <5%. Conclusions: It is suggested that these identified compounds may greatly contribute to the antioxidant capacity of C. hindsii as well as its potential pharmaceutical properties. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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