Improved preimplantation development of porcine somatic cell nuclear transfer embryos by caffeine treatment.

Autor:	Kim G; College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Korea.; Xenotransplantation Research Center, College of Medicine, Seoul National University, Seoul 03080, Korea., Roy PK; College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Korea., Fang X; College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Korea., Hassan BM; College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Korea., Cho J; College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Korea. cjki@cnu.ac.kr.
Jazyk:	angličtina
Zdroj:	Journal of veterinary science [J Vet Sci] 2019 May; Vol. 20 (3), pp. e31.
DOI:	10.4142/jvs.2019.20.e31
Abstrakt:	This study examined the effects of a caffeine treatment to improve nuclear reprogramming in porcine cloned embryos. Embryonic development and the expression of genes related to pluripotency ( POU5F1 , SOX2 , NANOG , and CDX2 ) were compared after caffeine supplementation during manipulation at different concentrations (0, 1.25, 2.5, and 5.0 mM) and after varying the delayed activation time (control, 1, 2, and 4 h) after fusion. Caffeine added to media during manipulation produced a higher rate of development to blastocysts in the 1.25 mM group than in the other concentration groups (22.8% vs. 16.1%, 16.2%, and 19.2%; p < 0.05). When caffeine was added during the 4 h delayed activation, the 1.25 mM caffeine concentration produced a significantly higher rate of development than those in the other 4 h-activation-delayed caffeine concentration groups (22.4% vs. 9.4%, 14.0%, and 11.1%; p < 0.05). On the other hand, no significant improvement over that in the control group was observed when caffeine was supplemented during both the manipulation period and delayed activation period (16.0% vs. 15.2%), respectively. The levels of POU5F1, SOX2, and NANOG expression in blastocysts were significantly higher in the delayed activation caffeine group (4 h, 1.25 mM) than in the control group (1 h, 0 mM; p < 0.05). In conclusion, a caffeine treatment at 1.25 mM during delayed activation for 4 h can improve the preimplantation development of porcine somatic cell nuclear transfer embryos by activating nuclear reprogramming. Competing Interests: The authors declare no conflicts of interest. (© 2019 The Korean Society of Veterinary Science.)
Databáze:	MEDLINE
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