| Autor: |
Sutikno; Department of Food Science and Human Nutrition, Michigan State University, East Lansing, Michigan, 48824-1224., Abouzied MM; Neogen Corp., 620 Lesher Place, Lansing, Michigan 48912-1509, USA., Azcona-Olivera JI; Department of Food Science and Human Nutrition, Michigan State University, East Lansing, Michigan, 48824-1224., Hart LP; Department of Botany and Plant Pathology, Michigan State University, East Lansing, Michigan, 48824-1224., Pestka JJ; Department of Food Science and Human Nutrition, Michigan State University, East Lansing, Michigan, 48824-1224. |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of food protection [J Food Prot] 1996 Jun; Vol. 59 (6), pp. 645-651. |
| DOI: |
10.4315/0362-028X-59.6.645 |
| Abstrakt: |
A specific sheep polyclonal antiserum was applied to the competitive direct enzyme-linked immunosorbent assay (CD-ELISA) of fumonisins in Fusarium cultures, fresh corn, animal feed, and human foods, and the results were related to fumonisin B 1 (FB 1 ) levels determined by liquid chromatography (LC). The limit of detection of FB 1 for the CD-ELISA was 0.1 ng/ml. Cross reactivity (defined as [ng/ml FB 1 required for 50% inhibition]/[ng/ml of analogue required for 50% inhibition] × 100) for the antiserum in the ELISA was 100, 24, and 30%, for FB 1 FB 2 , and FB 3 , respectively. FB 1 was detected in F. moniliforme cultures grown on corn (≤760 μg/g) but not in those for F. graminearum . Fumonisin estimates were 2.8-fold higher by CD-ELISA than FB 1 estimates by LC. Upon using the antiserum in a commercial CD-ELISA format (Veratox), mean recovery of FB 1 from spiked corn (0.1 to 3 μg/g)was 85.2 ± 25.3%, whereas the mean recovery by LC was 74.1 ± 6.2%. Analyses of 43 fresh corn, 28 animal feed, and 14 human food samples revealed that estimates of total fumonisins by the commercial ELISA were 2.9-, 2.2-, and 1.3-fold higher than FB 1 values determined by LC. For each sample type, the ELISA significantly correlated with LC ( r > 0.9; P < 0.05). Taken together, the results suggest that the polyclonal antibody-based CD-ELISA could be a reliable first-tier screening procedure for fumonisins in corn, animal feed, and human food when used in conjunction with LC confirmation. The differences between the two methods suggests the further presence of FB 2 and FB 3 or structurally related compounds. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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