Cell Boundary Confinement Sets the Size and Position of the E. coli Chromosome.

Autor:	Wu F; Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology, Van der Maasweg 9, 2629 HZ, Delft, the Netherlands; Division of Geological and Planetary Sciences, California Institute of Technology, 1200 E California Blvd, Pasadena, CA 91125, USA., Swain P; Indian Institute of Technology Hyderabad, Kandi, Sangareddy 502285, Telangana, India., Kuijpers L; Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology, Van der Maasweg 9, 2629 HZ, Delft, the Netherlands., Zheng X; Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology, Van der Maasweg 9, 2629 HZ, Delft, the Netherlands., Felter K; Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology, Van der Maasweg 9, 2629 HZ, Delft, the Netherlands., Guurink M; Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology, Van der Maasweg 9, 2629 HZ, Delft, the Netherlands., Solari J; Institute AMOLF, Science Park 104, 1098 XG, Amsterdam, the Netherlands., Jun S; Department of Physics, University of California San Diego, 9500 Gilman Dr. La Jolla, CA 92093, USA; Section of Molecular Biology, Division of Biological Sciences, University of California San Diego, 9500 Gilman Dr. La Jolla, CA 92093, USA., Shimizu TS; Institute AMOLF, Science Park 104, 1098 XG, Amsterdam, the Netherlands., Chaudhuri D; Institute of Physics, Sachivalaya Marg, Bhubaneswar 751005, India; Homi Bhabha National Institute, Anushaktinagar, Mumbai 400094, India., Mulder B; Institute AMOLF, Science Park 104, 1098 XG, Amsterdam, the Netherlands; Laboratory of Cell Biology, Wageningen University, Droevendaalsesteeg 1, 6708 PB Wageningen, the Netherlands. Electronic address: mulder@amolf.nl., Dekker C; Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology, Van der Maasweg 9, 2629 HZ, Delft, the Netherlands. Electronic address: c.dekker@tudelft.nl.
Jazyk:	angličtina
Zdroj:	Current biology : CB [Curr Biol] 2019 Jul 08; Vol. 29 (13), pp. 2131-2144.e4. Date of Electronic Publication: 2019 May 30.
DOI:	10.1016/j.cub.2019.05.015
Abstrakt:	Although the spatiotemporal structure of the genome is crucial to its biological function, many basic questions remain unanswered on the morphology and segregation of chromosomes. Here, we experimentally show in Escherichia coli that spatial confinement plays a dominant role in determining both the chromosome size and position. In non-dividing cells with lengths increased to 10 times normal, single chromosomes are observed to expand > 4-fold in size. Chromosomes show pronounced internal dynamics but exhibit a robust positioning where single nucleoids reside robustly at mid-cell, whereas two nucleoids self-organize at 1/4 and 3/4 positions. The cell-size-dependent expansion of the nucleoid is only modestly influenced by deletions of nucleoid-associated proteins, whereas osmotic manipulation experiments reveal a prominent role of molecular crowding. Molecular dynamics simulations with model chromosomes and crowders recapitulate the observed phenomena and highlight the role of entropic effects caused by confinement and molecular crowding in the spatial organization of the chromosome. (Copyright © 2019. Published by Elsevier Ltd.)
Databáze:	MEDLINE
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