Alternative method to improve the ethyl valerate yield using an immobilised Burkholderia cepacia lipase.

Autor: Moreira WC; a School of Applied Sciences/FCA, Research Group in Manufacturing of Advanced Materials (CPMMA), University of Campinas (UNICAMP) , Limeira , Sao Paulo , Brazil., Elias ALP; a School of Applied Sciences/FCA, Research Group in Manufacturing of Advanced Materials (CPMMA), University of Campinas (UNICAMP) , Limeira , Sao Paulo , Brazil., Osório WR; a School of Applied Sciences/FCA, Research Group in Manufacturing of Advanced Materials (CPMMA), University of Campinas (UNICAMP) , Limeira , Sao Paulo , Brazil.; b School of Technology, University of Campinas , Limeira , Sao Paulo , Brazil., Padilha GS; a School of Applied Sciences/FCA, Research Group in Manufacturing of Advanced Materials (CPMMA), University of Campinas (UNICAMP) , Limeira , Sao Paulo , Brazil.
Jazyk: angličtina
Zdroj: Journal of microencapsulation [J Microencapsul] 2019 Jun; Vol. 36 (4), pp. 327-337. Date of Electronic Publication: 2019 Jun 24.
DOI: 10.1080/02652048.2019.1626927
Abstrakt: This study is focussed on a biocatalysing chemical synthesis in order to produce a green apple flavour (ethyl valerate) using an immobilised lipase from Burkholderia cepacia . A strategy to improve the lipase stability during the esterification is used. In order to increase the ethyl valerate efficiency, an alternative method using the buffer pH to dissolve the lipase into alginate is proposed. Parameters of the immobilised lipase such as pH, temperature, activation energy and stirring speed are evaluated. The optimal condition using the substrate concentration and the lipase loading is provided. After 5 recyclability cycles, the immobilised lipase reveals a decreasing ∼25% in the ethyl valerate yield. An economical ester synthetising associated with the esterification efficiency is evidenced. This induces that a potential industrial application can be considered. This due to the demand for ethyl valerate in the flavour industry is required.
Databáze: MEDLINE
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