In vitro antiproliferative studies of extracts of the marine molluscs: Tympanatonus fuscatus Var radula (linnaeus) and Pachymelania aurita (muller).
| Autor: | Eghianruwa Q; Department of Molecular Biology and Biotechnology, Pan African University Institute of Science, Technology and Innovation Kenya., Osoniyi O; Department of Biochemistry and Molecular Biology, Obafemi Awolowo University Ile Ife, Nigeria., Wachira S; Kenya Medical Research Institute Nairobi, Kenya., Maina N; Department of Molecular Biology and Biotechnology, Pan African University Institute of Science, Technology and Innovation Kenya.; Biochemistry Department, Jomo Kenyatta University of Agriculture and Technology Juja, Kenya., Mbugua R; Kenya Medical Research Institute Nairobi, Kenya., Imbuga M; Biochemistry Department, Jomo Kenyatta University of Agriculture and Technology Juja, Kenya. |
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| Jazyk: | angličtina |
| Zdroj: | International journal of biochemistry and molecular biology [Int J Biochem Mol Biol] 2019 Apr 15; Vol. 10 (1), pp. 1-8. Date of Electronic Publication: 2019 Apr 15 (Print Publication: 2019). |
| Abstrakt: | This study aimed to investigate the antimitotic and antiproliferation activities of crude acetone-methanol and aqueous extracts of two marine molluscs commonly found in the Niger Delta region of Nigeria; T.fuscatus and P.aurita , against human cancerous cell lines (DU145, Hep-2, and HCC1395) cell lines in vitro . The antimitotic activity of the extracts was evaluated using Allium cepa root meristematic cells. Antiproliferative activity of the plant extracts against the cancerous cell lines was compared with normal cell line (VeroE6). Doxorubicin was used as a positive control. Gene expression studies using qPCR for the proapoptotic genes, CASP3, CASP8 and P53 were also carried out. The alcohol extract of T.fuscatus (TFAC) exhibited the most promising activity against all the cancer cell lines tested (DU145 IC Competing Interests: None. |
| Databáze: | MEDLINE |
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