Gut microbiota imbalances in Tunisian participants with type 1 and type 2 diabetes mellitus.

Autor:	Fassatoui M; Laboratory of Biomedical Genomics and Oncogenetics, Institut Pasteur de Tunis, 13, Place Pasteur, B.P.74, 1002, Belvédère, Tunis, Tunisia.; University of Tunis El Manar, Campus Universitaire Farhat Hached, BP n° 94, Rommana, 1068, Tunis, Tunisia., Lopez-Siles M; Laboratory of Molecular Microbiology, Biology Department, Universitat de Girona, Maria Aurèlia Capmany, 40, 17003, Girona, Spain., Díaz-Rizzolo DA; Diabetes and Obesity Research Laboratory, Institut d'Investigations Biomèdiques, August Pi I Sunyer (IDIBAPS), Hospital Clinic, Rosselló 149-153, 08036, Barcelona Spain., Jmel H; Laboratory of Biomedical Genomics and Oncogenetics, Institut Pasteur de Tunis, 13, Place Pasteur, B.P.74, 1002, Belvédère, Tunis, Tunisia., Naouali C; Laboratory of Biomedical Genomics and Oncogenetics, Institut Pasteur de Tunis, 13, Place Pasteur, B.P.74, 1002, Belvédère, Tunis, Tunisia.; University of Tunis El Manar, Campus Universitaire Farhat Hached, BP n° 94, Rommana, 1068, Tunis, Tunisia., Abdessalem G; Laboratory of Biomedical Genomics and Oncogenetics, Institut Pasteur de Tunis, 13, Place Pasteur, B.P.74, 1002, Belvédère, Tunis, Tunisia.; University of Tunis El Manar, Campus Universitaire Farhat Hached, BP n° 94, Rommana, 1068, Tunis, Tunisia., Chikhaoui A; Laboratory of Biomedical Genomics and Oncogenetics, Institut Pasteur de Tunis, 13, Place Pasteur, B.P.74, 1002, Belvédère, Tunis, Tunisia.; University of Tunis El Manar, Campus Universitaire Farhat Hached, BP n° 94, Rommana, 1068, Tunis, Tunisia., Nadal B; Diabetes and Obesity Research Laboratory, Institut d'Investigations Biomèdiques, August Pi I Sunyer (IDIBAPS), Hospital Clinic, Rosselló 149-153, 08036, Barcelona Spain., Jamoussi H; Laboratory of Biomedical Genomics and Oncogenetics, Institut Pasteur de Tunis, 13, Place Pasteur, B.P.74, 1002, Belvédère, Tunis, Tunisia.; Department of Nutritional Diseases A, National Institute of Nutrition and Food Technology, 11, Rue Djebel Lakhdar, Bab Saâdoun, 1007, Tunis, Tunisia., Abid A; Laboratory of Biomedical Genomics and Oncogenetics, Institut Pasteur de Tunis, 13, Place Pasteur, B.P.74, 1002, Belvédère, Tunis, Tunisia.; Department of External Consultation, National Institute of Nutrition and Food Technology, 11, Rue Djebel Lakhdar, Bab Saâdoun, 1007, Tunis, Tunisia., Gomis R; Diabetes and Obesity Research Laboratory, Institut d'Investigations Biomèdiques, August Pi I Sunyer (IDIBAPS), Hospital Clinic, Rosselló 149-153, 08036, Barcelona Spain., Abdelhak S; Laboratory of Biomedical Genomics and Oncogenetics, Institut Pasteur de Tunis, 13, Place Pasteur, B.P.74, 1002, Belvédère, Tunis, Tunisia.; University of Tunis El Manar, Campus Universitaire Farhat Hached, BP n° 94, Rommana, 1068, Tunis, Tunisia., Martinez-Medina M; Laboratory of Molecular Microbiology, Biology Department, Universitat de Girona, Maria Aurèlia Capmany, 40, 17003, Girona, Spain., Kefi R; Laboratory of Biomedical Genomics and Oncogenetics, Institut Pasteur de Tunis, 13, Place Pasteur, B.P.74, 1002, Belvédère, Tunis, Tunisia rym.kefi@pasteur.rns.tn.; University of Tunis El Manar, Campus Universitaire Farhat Hached, BP n° 94, Rommana, 1068, Tunis, Tunisia.
Jazyk:	angličtina
Zdroj:	Bioscience reports [Biosci Rep] 2019 Jun 18; Vol. 39 (6). Date of Electronic Publication: 2019 Jun 18 (Print Publication: 2019).
DOI:	10.1042/BSR20182348
Abstrakt:	Gut microbiota plays an important role in the regulation of the immune system and host's metabolism. We aimed to characterize the gut microbiota of Tunisian participants with and without diabetes.We enrolled ten participants with type 1 diabetes mellitus (T1DM), ten patients with type 2 diabetes mellitus (T2DM), and 11 subjects without diabetes. Bacteria was quantified in fecal samples by quantitative PCR (qPCR). Statistical tests and multivariate analysis were performed using RStudio program.Results showed that the proportions of Firmicutes, Akkermansia muciniphila , and Faecalibacterium prausnitzii ( P ≤0.041), as well as, the ratio Firmicutes/Bacteroidetes decreased in participants with T1DM compared with those without diabetes (p = 0.036). Participants with T2DM presented a reduction in the amounts of A. muciniphila and F. prausnitzii compared with those without diabetes ( P ≤0.036). Furthermore, A. muciniphila is negatively correlated with glucose level ( P =0.022) and glycated hemoglobin (HbA1c) ( P =0.035). Multivariate analysis revealed that participants with diabetes formed a cluster apart compared with those without diabetes.In conclusion the gut bacteria of Tunisian participants with diabetes was altered. The gut bacterial profile, especially the distribution of A muciniphila in participants with diabetes was affected by glycemic dysregulation. The investigation of the gut microbiota may help clinicians to improve diagnosis and treatment of diabetes and its complications. (© 2019 The Author(s).)
Databáze:	MEDLINE
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