Graphene quantum dots unraveling: Green synthesis, characterization, radiolabeling with 99mTc, in vivo behavior and mutagenicity.

Autor: de Menezes FD; Federal Institute of Education, Science and Technology of Pernambuco, Brazil., Dos Reis SRR; Brazilian Nuclear Energy Commission, Nuclear Engineering Institute, Rua Helio de Almeida 75, Ilha do Fundão, CEP 21941-614 Rio de Janeiro, Brazil., Pinto SR; Brazilian Nuclear Energy Commission, Nuclear Engineering Institute, Rua Helio de Almeida 75, Ilha do Fundão, CEP 21941-614 Rio de Janeiro, Brazil., Portilho FL; Brazilian Nuclear Energy Commission, Nuclear Engineering Institute, Rua Helio de Almeida 75, Ilha do Fundão, CEP 21941-614 Rio de Janeiro, Brazil., do Vale Chaves E Mello F; Brazilian Nuclear Energy Commission, Nuclear Engineering Institute, Rua Helio de Almeida 75, Ilha do Fundão, CEP 21941-614 Rio de Janeiro, Brazil., Helal-Neto E; Brazilian Nuclear Energy Commission, Nuclear Engineering Institute, Rua Helio de Almeida 75, Ilha do Fundão, CEP 21941-614 Rio de Janeiro, Brazil., da Silva de Barros AO; Brazilian Nuclear Energy Commission, Nuclear Engineering Institute, Rua Helio de Almeida 75, Ilha do Fundão, CEP 21941-614 Rio de Janeiro, Brazil., Alencar LMR; Federal University of Maranhão, Department of Physics, Avenida dos Portugueses 500, Vila Bacanga, CEP 65080-805 São Luís, Maranhão, Brazil., de Menezes AS; Federal University of Maranhão, Department of Physics, Avenida dos Portugueses 500, Vila Bacanga, CEP 65080-805 São Luís, Maranhão, Brazil., Dos Santos CC; Federal University of Maranhão, Department of Physics, Avenida dos Portugueses 500, Vila Bacanga, CEP 65080-805 São Luís, Maranhão, Brazil., Saraiva-Souza A; Federal University of Piaui, Department of Physics, Bairro Ininga, CEP: 64.049-550 Teresina, Piaui, Brazil., Perini JA; Research Laboratory of Pharmaceutical Sciences, Zona Oeste State University, Avenida Manuel Caldeira de Alvarenga 1.203, CEP 23070-200 Campo Grande, Rio de Janeiro, Brazil., Machado DE; Research Laboratory of Pharmaceutical Sciences, Zona Oeste State University, Avenida Manuel Caldeira de Alvarenga 1.203, CEP 23070-200 Campo Grande, Rio de Janeiro, Brazil., Felzenswalb I; Department of Biophysics and Biometry, Rio de Janeiro State University, Boulevard 28 de Setembro, 87 Fundos, 4 ° Andar, CEP 20551-030 Rio de Janeiro, RJ, Brazil., Araujo-Lima CF; Department of Biophysics and Biometry, Rio de Janeiro State University, Boulevard 28 de Setembro, 87 Fundos, 4 ° Andar, CEP 20551-030 Rio de Janeiro, RJ, Brazil., Sukhanova A; Laboratoire de Recherche en Nanosciences (LRN-EA4682), Université de Reims Champagne-Ardenne, 51, rue Cognacq Jay, 51096 Reims, France; Laboratory of Nano-Bioengineering, National Research Nuclear University MEPhI (Moscow Engineering Physics Institute), KashirskoyeShosse 31, 115409 Moscow, Russian Federation., Nabiev I; Laboratoire de Recherche en Nanosciences (LRN-EA4682), Université de Reims Champagne-Ardenne, 51, rue Cognacq Jay, 51096 Reims, France; Laboratory of Nano-Bioengineering, National Research Nuclear University MEPhI (Moscow Engineering Physics Institute), KashirskoyeShosse 31, 115409 Moscow, Russian Federation., Santos-Oliveira R; Brazilian Nuclear Energy Commission, Nuclear Engineering Institute, Rua Helio de Almeida 75, Ilha do Fundão, CEP 21941-614 Rio de Janeiro, Brazil; Zona Oeste State University, Laboratory of Radiopharmacy and Nanoradiopharmaceuticals, Campo Grande, Rio de Janeiro, Brazil. Electronic address: roliveira@ien.gov.br.
Jazyk: angličtina
Zdroj: Materials science & engineering. C, Materials for biological applications [Mater Sci Eng C Mater Biol Appl] 2019 Sep; Vol. 102, pp. 405-414. Date of Electronic Publication: 2019 Apr 24.
DOI: 10.1016/j.msec.2019.04.058
Abstrakt: Graphene is one of the crystalline forms of carbon, along with diamond, graphite, carbon nanotubes, and fullerenes, and is considered as a revolutionary and innovating product. The use of a graphene-based nanolabels is one of the latest and most prominent application of graphene, especially in the field of diagnosis and, recently, in loco radiotherapy when coupled with radioisotopes. However, its biological behavior and mutagenicity in different cell or animal models, as well as the in vivo functional activities, are still unrevealed. In this study we have developed by a green route of synthesizing graphene quantum dots (GQDs) and characterized them. We have also developed a methodology for direct radiolabeling of GQDs with radioisotopes.Finally; we have evaluated in vivo biological behavior of GQDs using two different mice models and tested in vitro mutagenicity of GQDs. The results have shown that GQDs were formed with a size range of 160-280 nm, which was confirmed by DRX and Raman spectroscopy analysis, corroborating that the green synthesis is an alternative, environmentally friendly way to produce graphene. The radiolabeling test has shown that stable radiolabeled GQDs can be produced with a high yield (>90%). The in vivo test has demonstrated a ubiquitous behavior when administered to healthy animals, with a high uptake by liver (>26%) and small intestine (>25%). Otherwise, in an inflammation/VEGF hyperexpression animal model (endometriosis), a very peculiar behavior of GQDs was observed, with a high uptake by kidneys (over 85%). The mutagenicity test has demonstrated A:T to G:C substitutions suggesting that GQDs exhibits mutagenic activity.
(Copyright © 2019 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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