Multi-Walled Carbon Nanotube-Induced Gene Expression Biomarkers for Medical and Occupational Surveillance.

Autor: Snyder-Talkington BN; West Virginia University Cancer Institute, West Virginia University, Morgantown, WV 26506, USA. brandi@branditalkington.com., Dong C; West Virginia University Cancer Institute, West Virginia University, Morgantown, WV 26506, USA. lindadong2004@yahoo.com., Singh S; West Virginia University Cancer Institute, West Virginia University, Morgantown, WV 26506, USA. ss0083@mix.wvu.edu., Raese R; West Virginia University Cancer Institute, West Virginia University, Morgantown, WV 26506, USA. rebecca.raese@gmail.com., Qian Y; National Institute for Occupational and Environmental Safety and Health, 1095 Willowdale Rd., Morgantown, WV 26505, USA. yaq2@cdc.gov., Porter DW; National Institute for Occupational and Environmental Safety and Health, 1095 Willowdale Rd., Morgantown, WV 26505, USA. dhp7@cdc.gov., Wolfarth MG; National Institute for Occupational and Environmental Safety and Health, 1095 Willowdale Rd., Morgantown, WV 26505, USA. mgz1@cdc.gov., Guo NL; West Virginia University Cancer Institute, West Virginia University, Morgantown, WV 26506, USA. lguo@hsc.wvu.edu.; Department of Occupational and Environmental Health Sciences, School of Public Health, West Virginia University, Morgantown, WV 26506, USA. lguo@hsc.wvu.edu.
Jazyk: angličtina
Zdroj: International journal of molecular sciences [Int J Mol Sci] 2019 May 29; Vol. 20 (11). Date of Electronic Publication: 2019 May 29.
DOI: 10.3390/ijms20112635
Abstrakt: As the demand for multi-walled carbon nanotube (MWCNT) incorporation into industrial and biomedical applications increases, so does the potential for unintentional pulmonary MWCNT exposure, particularly among workers during manufacturing. Pulmonary exposure to MWCNTs raises the potential for development of lung inflammation, fibrosis, and cancer among those exposed; however, there are currently no effective biomarkers for detecting lung fibrosis or predicting the risk of lung cancer resulting from MWCNT exposure. To uncover potential mRNAs and miRNAs that could be used as markers of exposure, this study compared in vivo mRNA and miRNA expression in lung tissue and blood of mice exposed to MWCNTs with in vitro mRNA and miRNA expression from a co-culture model of human lung epithelial and microvascular cells, a system previously shown to have a higher overall genome-scale correlation with mRNA expression in mouse lungs than either cell type grown separately. Concordant mRNAs and miRNAs identified by this study could be used to drive future studies confirming human biomarkers of MWCNT exposure. These potential biomarkers could be used to assess overall worker health and predict the occurrence of MWCNT-induced diseases.
Competing Interests: The authors declare no conflict of interest.
Databáze: MEDLINE
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