Evaluation of the interaction between polymyxin B and Pseudomonas aeruginosa biofilm and planktonic cells: reactive oxygen species induction and zeta potential.

Autor: Lima MR; Faculdade de Ciências da Saúde, Universidade Vale do Rio Doce, Governador Valadares, MG, Brazil., Ferreira GF; Departamento de Farmácia, Programa Multicêntrico de Pós-Graduação em Bioquímica e Biologia Molecular, Universidade Federal de Juiz de Fora, UFJF, Campus Governador Valadares - MG. R. Manoel Byrro, 241 - Vila Bretas, Governador Valadares, MG, 35032-620, Brazil. gabriella.freitas@ufjf.edu.br., Nunes Neto WR; Universidade CEUMA, São Luís, MA, Brazil., Monteiro JM; Universidade CEUMA, São Luís, MA, Brazil., Santos ÁRC; Departamento de Farmácia, Programa Multicêntrico de Pós-Graduação em Bioquímica e Biologia Molecular, Universidade Federal de Juiz de Fora, UFJF, Campus Governador Valadares - MG. R. Manoel Byrro, 241 - Vila Bretas, Governador Valadares, MG, 35032-620, Brazil., Tavares PB; Faculdade de Ciências da Saúde, Universidade Vale do Rio Doce, Governador Valadares, MG, Brazil., Denadai ÂML; Departamento de Farmácia, Programa Multicêntrico de Pós-Graduação em Bioquímica e Biologia Molecular, Universidade Federal de Juiz de Fora, UFJF, Campus Governador Valadares - MG. R. Manoel Byrro, 241 - Vila Bretas, Governador Valadares, MG, 35032-620, Brazil., Bomfim MRQ; Universidade CEUMA, São Luís, MA, Brazil., Dos Santos VL; Departamento de Microbiologia, Instituto de Ciência Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil., Marques SG; Hospital Universitário da Universidade Federal do Maranhão, São Luís, MA, Brazil.; Laboratório Cedro, São Luís, MA, Brazil., de Souza Monteiro A; Universidade CEUMA, São Luís, MA, Brazil.
Jazyk: angličtina
Zdroj: BMC microbiology [BMC Microbiol] 2019 May 29; Vol. 19 (1), pp. 115. Date of Electronic Publication: 2019 May 29.
DOI: 10.1186/s12866-019-1485-8
Abstrakt: Background: Although the most widely accepted mechanism of action for polymyxins is related to bacterial lysis via disruption, we hypothesized that this antimicrobial drug class could have other effects on Pseudomonas aeruginosa planktonic and sessile cells. Little is known regarding oxidative burst and zeta potential (ZP) data associated with the interaction between polymyxin B and P. aeruginosa cells. The present study evaluated endogenous reactive oxygen species (ROS) production and changes in the net charges of biofilm and planktonic cells in response to polymyxin B.
Results: Polymyxin B induced concentration-dependent killing at all concentrations tested in planktonic and sessile cells from P. aeruginosa strains. Sublethal concentrations of polymyxin B induced oxidative burst. ROS production was higher in resistant planktonic cells than in biofilm cells but this was not observed for susceptible cells. Moreover, no net surface charge alterations were observed in planktonic cells from a susceptible strain treated with polymyxin B, but a significant increase of ZP was noted in planktonic cells from a resistant strain.
Conclusion: Oxidative burst generated by planktonic and sessile cells from P. aeruginosa strains against polymyxin B indicates that ROS may have an important role in the mechanism of action of this drug. ZP data revealed that electrostatic interactions of the cationic peptide with the anionic surface of the cells are strain-dependent. Therefore, we suggested that the intracellular effects of polymyxin B should be further investigated to understand polymyxin B-induced stress in P. aeruginosa.
Databáze: MEDLINE
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