Methadone enhances the effectiveness of 5-aminolevulinic acid-based photodynamic therapy for squamous cell carcinoma and glioblastoma in vitro.

Autor: Shi L; Laser-Forschungslabor, LIFE Center, University Hospital, LMU Munich, Munich, Germany.; Institute of Photomedicine, Shanghai Skin Disease Hospital, Tongji University School of Medicine, Shanghai, People's Republic of China., Buchner A; Labor für Tumorimmunologie, LIFE Center, University Hospital, LMU Munich, Munich, Germany.; Department of Urology, University Hospital, LMU Munich, Munich, Germany., Pohla H; Labor für Tumorimmunologie, LIFE Center, University Hospital, LMU Munich, Munich, Germany.; Department of Urology, University Hospital, LMU Munich, Munich, Germany., Pongratz T; Laser-Forschungslabor, LIFE Center, University Hospital, LMU Munich, Munich, Germany.; Department of Urology, University Hospital, LMU Munich, Munich, Germany., Rühm A; Laser-Forschungslabor, LIFE Center, University Hospital, LMU Munich, Munich, Germany.; Department of Urology, University Hospital, LMU Munich, Munich, Germany., Zimmermann W; Labor für Tumorimmunologie, LIFE Center, University Hospital, LMU Munich, Munich, Germany.; Department of Urology, University Hospital, LMU Munich, Munich, Germany., Gederaas OA; Department of Chemistry, Norwegian University of Science and Technology, NTNU, Trondheim, Norway.; Odrun Arna Gederaas, Department of Clinical and Molecular Medicine, Norwegian University of Science and Technology, NTNU, Trondheim, Norway., Zhang L; Institute of Photomedicine, Shanghai Skin Disease Hospital, Tongji University School of Medicine, Shanghai, People's Republic of China., Wang X; Institute of Photomedicine, Shanghai Skin Disease Hospital, Tongji University School of Medicine, Shanghai, People's Republic of China., Stepp H; Laser-Forschungslabor, LIFE Center, University Hospital, LMU Munich, Munich, Germany.; Department of Urology, University Hospital, LMU Munich, Munich, Germany., Sroka R; Laser-Forschungslabor, LIFE Center, University Hospital, LMU Munich, Munich, Germany.; Institute of Photomedicine, Shanghai Skin Disease Hospital, Tongji University School of Medicine, Shanghai, People's Republic of China.; Department of Urology, University Hospital, LMU Munich, Munich, Germany.
Jazyk: angličtina
Zdroj: Journal of biophotonics [J Biophotonics] 2019 Oct; Vol. 12 (10), pp. e201800468. Date of Electronic Publication: 2019 Jul 04.
DOI: 10.1002/jbio.201800468
Abstrakt: Although having shown promising clinical outcomes, the effectiveness of 5-aminolevulinic acid-based photodynamic therapy (ALA-PDT) for squamous cell carcinoma (SCC) and glioblastoma remains to be improved. The analgesic drug methadone is able to sensitize various tumors to chemotherapy. In this in vitro study, the influence of methadone to the effectiveness of ALA-PDT for SCC (FADU) and glioblastoma (A172) was investigated on the protoporphyrin IX (PpIX) fluorescence, survival rates, apoptosis, and cell cycle phase, each with or without the presence of methadone. The production of PpIX was increased by methadone in FADU cells while it was decreased in A172 cells. The survival rates of both cell lines treated by ALA-PDT were significantly reduced by the combination with methadone (P < .05). Methadone also significantly increased the percentage of apoptotic cells and improved the effect of ALA-PDT on the cell cycle phase arrest in the G0/G1 phase (P < .05). This study demonstrates the potential of methadone to influence the cytotoxic effect of ALA-PDT for both SCC and glioblastoma cell lines.
(© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
Databáze: MEDLINE
Externí odkaz: