Emergence of Carbapenem-Resistant Pseudomonas asiatica Producing NDM-1 and VIM-2 Metallo-β-Lactamases in Myanmar.

Autor: Tohya M; Department of Microbiology, Juntendo University School of Medicine, Tokyo, Japan., Tada T; Department of Microbiology, Juntendo University School of Medicine, Tokyo, Japan., Watanabe S; Department of Microbiome Research, Juntendo University School of Medicine, Tokyo, Japan., Kuwahara-Arai K; Department of Microbiology, Juntendo University School of Medicine, Tokyo, Japan., Zin KN; Clinical Pathology Department, Yangon General Hospital, Yangon, Myanmar., Zaw NN; Clinical Pathology Section, Mandalay General Hospital, Mandalay, Myanmar., Aung MY; Clinical Pathology Section, Thingangyun San Pya General Hospital, Yangon, Myanmar., Mya S; National Health Laboratory, Yangon, Myanmar., Zan KN; National Health Laboratory, Yangon, Myanmar., Kirikae T; Department of Microbiology, Juntendo University School of Medicine, Tokyo, Japan t-kirikae@juntendo.ac.jp., Tin HH; National Health Laboratory, Yangon, Myanmar.
Jazyk: angličtina
Zdroj: Antimicrobial agents and chemotherapy [Antimicrob Agents Chemother] 2019 Jul 25; Vol. 63 (8). Date of Electronic Publication: 2019 Jul 25 (Print Publication: 2019).
DOI: 10.1128/AAC.00475-19
Abstrakt: Pseudomonas asiatica is a recently proposed species of the genus Pseudomonas This study describes eight isolates of carbapenem-resistant P. asiatica harboring bla NDM-1 and bla VIM-2 , genes encoding metallo-β-lactamase (MBL). These isolates were obtained from urine samples of patients hospitalized in Myanmar. These isolates were resistant to carbapenems but susceptible to colistin. All eight isolates were positive for a carbapenemase inactivation method, CIMTrisII, and seven were positive on an immunochromatographic assay for NDM-type MBL. One isolate was highly resistant to aminoglycosides. Whole-genome sequencing showed that seven isolates harbored bla NDM-1 and one harbored bla VIM-2 , with these genes located on the chromosome. One isolate harbored bla NDM-1 and rmtC , a gene encoding 16S rRNA methylase. Five types of genomic environments surrounding bla NDM-1 and bla VIM-2 were detected in these eight isolates, with four isolates having the same type. These data indicate that P. asiatica isolates harboring genes encoding carbapenemases, including bla NDM-1 and bla VIM-2 , are spreading in medical settings in Myanmar.
(Copyright © 2019 American Society for Microbiology.)
Databáze: MEDLINE
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