miR551b Regulates Colorectal Cancer Progression by Targeting the ZEB1 Signaling Axis.

Autor: Kim KS; Soonchunhyang Institute of Medi-bio Science (SIMS), Soonchunhyang University, Cheonan-si 31151, Korea. kimks5005gt@gmail.com., Jeong D; Department of Pathology, College of Medicine, Soonchunhyang University, Cheonan-si 31151, Korea. juny1024@sch.ac.kr.; Soonchunhyang Medical Science Research Institute, College of Medicine, Soonchunhyang University, Cheonan-si 31151, Korea. juny1024@sch.ac.kr., Sari IN; Soonchunhyang Institute of Medi-bio Science (SIMS), Soonchunhyang University, Cheonan-si 31151, Korea. itanov07@gmail.com., Wijaya YT; Soonchunhyang Institute of Medi-bio Science (SIMS), Soonchunhyang University, Cheonan-si 31151, Korea. yoseph.toni@gmail.com., Jun N; Soonchunhyang Institute of Medi-bio Science (SIMS), Soonchunhyang University, Cheonan-si 31151, Korea. jny0407@naver.com., Lee S; Soonchunhyang Institute of Medi-bio Science (SIMS), Soonchunhyang University, Cheonan-si 31151, Korea. october-92@daum.net., Yang YG; Soonchunhyang Institute of Medi-bio Science (SIMS), Soonchunhyang University, Cheonan-si 31151, Korea. syyangyinggui@163.com., Lee SH; Liver Clinic, Soonchunhyang University Cheonan Hospital, Cheonan-si 31151, Korea. stevesh@schmc.ac.kr., Kwon HY; Soonchunhyang Institute of Medi-bio Science (SIMS), Soonchunhyang University, Cheonan-si 31151, Korea. hykwon@sch.ac.kr.
Jazyk: angličtina
Zdroj: Cancers [Cancers (Basel)] 2019 May 27; Vol. 11 (5). Date of Electronic Publication: 2019 May 27.
DOI: 10.3390/cancers11050735
Abstrakt: Our current understanding of the role of microRNA 551b (miR551b) in the progression of colorectal cancer (CRC) remains limited. Here, studies using both ectopic expression of miR551b and miR551b mimics revealed that miR551b exerts a tumor suppressive effect in CRC cells. Specifically, miR551b was significantly downregulated in both patient-derived CRC tissues and CRC cell lines compared to normal tissues and non-cancer cell lines. Also, miR551b significantly inhibited the motility of CRC cells in vitro, including migration, invasion, and wound healing rates, but did not affect cell proliferation. Mechanistically, miR551b targets and inhibits the expression of ZEB1 (Zinc finger E-box-binding homeobox 1), resulting in the dysregulation of EMT (epithelial-mesenchymal transition) signatures. More importantly, miR551b overexpression was found to reduce the tumor size in a xenograft model of CRC cells in vivo. Furthermore, bioinformatic analyses showed that miR551b expression levels were markedly downregulated in the advanced-stage CRC tissues compared to normal tissues, and ZEB1 was associated with the disease progression in CRC patients. Our findings indicated that miR551b could serve as a potential diagnostic biomarker and could be utilized to improve the therapeutic outcomes of CRC patients.
Competing Interests: The authors declare no conflict of interest.
Databáze: MEDLINE
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