Proteomic markers of low and high fertility bovine spermatozoa separated by Percoll gradient.

Autor: D'Amours O; Département d'obstétrique, Gynécologie et Reproduction, Centre de Recherche du Centre Hospitalier de l'Université Laval, Québec, Québec, Canada., Calvo É; Proteomic Core Facility, Centre de Recherche du Centre Hospitalier de l'Université Laval, Axe Reproduction, Santé de la mère et de l'enfant, Québec, Québec, Canada., Bourassa S; Proteomic Core Facility, Centre de Recherche du Centre Hospitalier de l'Université Laval, Axe Reproduction, Santé de la mère et de l'enfant, Québec, Québec, Canada., Vincent P; Department of Research and Development, Semex Alliance, L'Alliance Boviteq Inc, Saint-Hyacinthe, Québec, Canada., Blondin P; Department of Research and Development, Semex Alliance, L'Alliance Boviteq Inc, Saint-Hyacinthe, Québec, Canada., Sullivan R; Département d'obstétrique, Gynécologie et Reproduction, Centre de Recherche du Centre Hospitalier de l'Université Laval, Québec, Québec, Canada.
Jazyk: angličtina
Zdroj: Molecular reproduction and development [Mol Reprod Dev] 2019 Aug; Vol. 86 (8), pp. 999-1012. Date of Electronic Publication: 2019 May 27.
DOI: 10.1002/mrd.23174
Abstrakt: In the context of artificial insemination, male fertility is defined as the ability to produce functional spermatozoa able to withstand cryopreservation. We hypothesized that interindividual variations in fertility depend on the proportion of the fully functional sperm population contained in the insemination dose. The objective of this study was to identify protein markers of the fully functional sperm subpopulation. Insemination doses from four high-fertility (HF) and four low-fertility (LF) bulls with comparable post-thaw quality parameters were selected for proteomic analysis using iTRAQ technology. Thawed semen was centrifuged through a Percoll gradient to segregate the motile (high density [HD]) from the immotile (low density [LD]) sperm populations. Sperm proteins were extracted with sodium deoxycholate and four groups were compared: LD and HD spermatozoa from LF and HF bulls. A total of 498 unique proteins were identified and quantified. Comparison of HD spermatozoa from HF and LF bulls revealed that five proteins were significantly more abundant in the HF group (AK8, TPI1, TSPAN8, OAT, and DBIL5) whereas five proteins were more abundant in the LF group (RGS22, ATP5J, CLU, LOC616319, and CCT5). Comparison of LD spermatozoa from HF and LF bulls revealed that four proteins were significantly more abundant in the HF group (IL4I1, CYLC2, OAT, and ARMC3) whereas 15 proteins were significantly more abundant in the LF group (HADHA, HSP90AA1, DNASE1L3, SLC25A20, GPX5, TCP1, HIP1, CLU, G5E622, LOC616319, HSPA2, NUP155, DPY19L2, SPERT, and SERPINE2). DBIL5, TSPAN8, and TPI1 showed potential as putative markers of the fully functional sperm subpopulation.
(© 2019 Wiley Periodicals, Inc.)
Databáze: MEDLINE
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