Potentiometric polyphenol oxidase biosensor for sensitive determination of phenolic micropollutant in environmental samples.

Autor: Yashas SR; Department of Environmental Engineering, JSS Science and Technology University, Mysuru, Karnataka, 570006, India., Sandeep S; Department of Chemistry, JSS Science and Technology University, Mysuru, Karnataka, 570006, India., Shivakumar BP; Department of Environmental Engineering, JSS Science and Technology University, Mysuru, Karnataka, 570006, India., Swamy NK; Department of Chemistry, JSS Science and Technology University, Mysuru, Karnataka, 570006, India. kumaryagati@gmail.com.
Jazyk: angličtina
Zdroj: Environmental science and pollution research international [Environ Sci Pollut Res Int] 2020 Aug; Vol. 27 (22), pp. 27234-27243. Date of Electronic Publication: 2019 May 27.
DOI: 10.1007/s11356-019-05495-2
Abstrakt: The present study demonstrates the development of polyphenol oxidase (PPO) biosensor for the detection of catechol using strontium copper oxide (SrCuO 2 ) and polypyrrole nanotubes (PPyNT) matrix. The SrCuO 2 micro-seeds, a perovskite compound, are synthesized by co-precipitation under pH 8.0. The as-synthesized micro-seeds are characterized by scanning electron microscopy (SEM), field emission scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDX), and X-ray diffraction spectroscopy (XRD). The proposed sensor is fabricated on pencil graphite (P-Gr) by successive deposition of PPyNT, SrCuO 2 , and PPO enzyme. The developed PPO/SrCuO 2 /PPyNT/P-Gr sensor is characterized by cyclic voltammetry (CV), differential pulse voltammetry (DPV), and electrochemical impedance spectroscopy (EIS) techniques. The PPO/SrCuO 2 /PPyNT/P-Gr displayed excellent electrocatalytic activity towards the oxidation and detection of catechol. The as-developed sensor showed sensitive response ascribing to limit of detection (LOD) of 0.15 μM and sensitivity of 15.60 μA μM -1  cm -2 . The fabricated sensor exhibited excellent repeatability and longer shelf life. The proposed biosensor finds its application within the broad linear range of 1-50 μM. Real sample analysis of mineral water, tap water, and domestic wastewater using developed sensor showed acceptable recovery. Hence, the biosensor endeavors its application in environmental monitoring and protection.
Databáze: MEDLINE
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