Viability of Equine Chondrocytes After Exposure to Mepivacaine and Ropivacaine In Vitro.

Autor: Silva GB; Department of Large Animal Clinics, School of Veterinary Medicine, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil. Electronic address: gabibiavaschi@hotmail.com., De La Côrte FD; Department of Large Animal Clinics, School of Veterinary Medicine, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil., Brass KE; Department of Large Animal Clinics, School of Veterinary Medicine, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil., Palma HE; Department of Small Animal Clinics, School of Veterinary Medicine, University of Cruz Alta, Cruz Alta, Rio Grande do Sul, Brazil., Gallio M; Department of Large Animal Clinics, School of Veterinary Medicine, Instituto de Desenvolvimento Educacional do Alto Uruguai, Getúlio Vargas, Rio Grande do Sul, Brazil., Cantarelli C; Department of Large Animal Clinics, School of Veterinary Medicine, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil., Bertolin K; Department of Large Animal Clinics, School of Veterinary Medicine, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil., Krause A; Department of Small Animal Clinics, School of Veterinary Medicine, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil., Wergutz J; Health Department, School of Biomedicine, Universidade Franciscana, Santa Maria, Rio Grande do Sul, Brazil., Fontanari Krause LM; Health Department, School of Biomedicine, Universidade Franciscana, Santa Maria, Rio Grande do Sul, Brazil., Antoniazzi AQ; Department of Large Animal Clinics, School of Veterinary Medicine, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil.
Jazyk: angličtina
Zdroj: Journal of equine veterinary science [J Equine Vet Sci] 2019 Jun; Vol. 77, pp. 80-85. Date of Electronic Publication: 2019 Mar 06.
DOI: 10.1016/j.jevs.2019.02.019
Abstrakt: Chondrocyte health is altered when exposed to local anesthetics, raising concerns as to the long-term effects of local anesthetics intra-articularly for diagnosis and analgesia. To investigate the drug with the lowest toxic potential, the effect of ropivacaine and mepivacaine on chondrocytes was evaluated. Articular cartilage from normal metacarpophalangeal joints of five equine cadaver specimens was used to establish chondrocyte cultures. Following seven days, chondrocytes were exposed to standard culture medium (DMEM), ropivacaine 7.5 mg/ml (ROP7.5), ropivacaine 10 mg/ml (ROP10), mepivacaine 20 mg/ml (MEP20), mepivacaine 30 mg/ml (MEP 30), and 0.9% saline solution (SAL). Chondrocyte viability was evaluated by trypan blue exclusion, MTT, and flow cytometry via cellular staining with propidium iodide. No differences were observed between treatments following trypan blue exclusion assay. A difference was observed between DMEM and all other treatment groups (P < .0001) with a significant viability drop using the MTT assay. Mepivacaine 20 mg/ml and MEP30 exposure between showed greatest decrease in cellular viability compared to SAL, ROP7.5, and ROP10 (P < .0001). Cellular viability decreased as measured by flow cytometry in all groups compared to DMEM and ROP7.5 (P < .02). Interestingly, the trypan blue, MTT, and flow cytometry assays yielded different results. Although there was no difference using trypan blue, MTT demonstrated that ropivacaine-treated cells had lower viability than DMEM, and cytometry found that ROP7.5 did not differ from DMEM. Results in vitro suggest that short-term exposure to ropivacaine may result in less chondrotoxicity than mepivacaine. In vivo studies are warranted investigating long-term effects of local anesthetics on equine articular cartilage.
(Copyright © 2019 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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