Mitogen-Induced Interferon Gamma Production in Human Whole Blood: The Effect of Heat and Cations.

Autor:	Nam JH; Department of Research and Development, The Korean Institute of Tuberculosis, 168-5 Osongsaengmyeong4-ro, Heungdeok-gu, Cheongju-si, Chungcheongbuk-do 28158, Korea.; Division of Antimicrobial Resistance, Center for Infectious Disease Research, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, 187 Osongsaengmyeong2-ro, Heungdeok-gu, Cheongju-si, Chungcheongbuk-do 28159, Korea., Cha B; Department of Research and Development, The Korean Institute of Tuberculosis, 168-5 Osongsaengmyeong4-ro, Heungdeok-gu, Cheongju-si, Chungcheongbuk-do 28158, Korea., Park JY; Molecular and Cellular Glycobiology Unit, Department of Biological Sciences, SungKyunKwan University, Seoburo, Jangan-Gu, Kyunggi-Do 16419, Korea., Abekura F; Molecular and Cellular Glycobiology Unit, Department of Biological Sciences, SungKyunKwan University, Seoburo, Jangan-Gu, Kyunggi-Do 16419, Korea., Kim CH; Molecular and Cellular Glycobiology Unit, Department of Biological Sciences, SungKyunKwan University, Seoburo, Jangan-Gu, Kyunggi-Do 16419, Korea., Kim JR; Department of Research and Development, The Korean Institute of Tuberculosis, 168-5 Osongsaengmyeong4-ro, Heungdeok-gu, Cheongju-si, Chungcheongbuk-do 28158, Korea.
Jazyk:	angličtina
Zdroj:	Current pharmaceutical biotechnology [Curr Pharm Biotechnol] 2019; Vol. 20 (7), pp. 562-572.
DOI:	10.2174/1389201020666190528093432
Abstrakt:	Background: Interferon-gamma release assays (IGRAs) are blood tests used to measure the amount of interferon-γ (IFN-γ) released by T lymphocytes after stimulation by antigens specific for the diagnosis of latent tuberculosis infection. A mitogen serves as a positive control to assess the immune function in IGRAs. Methods: This in vitro study was conducted to evaluate IFN-γ production by human whole blood stimulated with heat-treated and/or cation-supplemented phytohemagglutinin (PHA), concanavalin A (Con A) and pokeweed mitogen (PWM), using QuantiFERON-TB Gold Kit ELISA tests. Results: The optimal concentrations of PWM, Con A and PHA for IGRAs were 2 µg/mL, 5 µg/mL and 10 µg/mL, respectively. The results showed that IFN-γ production in response to PWM was the highest and PHA was the lowest amount. The median values of three mitogens were in the following order: PWM≥Con A≥ positive control>>PHA-P>>negative control. PWM and PHA were heat stable, while Con A was heat sensitive. The mitogen response of lymphocytes to untreated or heat-treated PWM and heat-treated Con A was increased in 1 mM Ca2+-supplemented groups, whereas the response to heat-treated PHA was decreased. Exposure to 1 mM Mg2+ had no effect on untreated or heat-treated PWM, and a concentration of 1 mM Zn2+ inhibited the stimulation of un-treated PWM. We found that calcium supplementation improved the PWM-induced production of IFN-γ. Conclusion: Therefore, PWM is an appropriate mitogen for use as a positive control in IGRAs. It is a potential indicator of cytokine production in the diagnostic as well as research settings, and calcium supplementation improved stimulation. (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.)
Databáze:	MEDLINE
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