Osteogenic potential of hexane and dichloromethane fraction of Cissus quadrangularis on murine preosteoblast cell line MC3T3-E1 (subclone 4).

Autor: Toor RH; School of Biological Sciences, Quaid-i-Azam Campus, University of the Punjab, Lahore, Pakistan., Malik S; School of Biological Sciences, Quaid-i-Azam Campus, University of the Punjab, Lahore, Pakistan., Qamar H; School of Biological Sciences, Quaid-i-Azam Campus, University of the Punjab, Lahore, Pakistan., Batool F; School of Biological Sciences, Quaid-i-Azam Campus, University of the Punjab, Lahore, Pakistan., Tariq M; School of Biological Sciences, Quaid-i-Azam Campus, University of the Punjab, Lahore, Pakistan., Nasir Z; School of Biological Sciences, Quaid-i-Azam Campus, University of the Punjab, Lahore, Pakistan., Tassaduq R; Department of Biotechnology, Kinnaird College for Women, Lahore, Punjab, Pakistan., Lian JB; Department of Biochemistry, University of Vermont College of Medicine, Burlington, Vermont., Stein JL; Department of Biochemistry, University of Vermont College of Medicine, Burlington, Vermont., Stein GS; Department of Biochemistry, University of Vermont College of Medicine, Burlington, Vermont., Shakoori AR; School of Biological Sciences, Quaid-i-Azam Campus, University of the Punjab, Lahore, Pakistan.; Department of Biochemistry, Faculty of Life Sciences, University of Central Punjab, Lahore, Pakistan.
Jazyk: angličtina
Zdroj: Journal of cellular physiology [J Cell Physiol] 2019 Dec; Vol. 234 (12), pp. 23082-23096. Date of Electronic Publication: 2019 May 26.
DOI: 10.1002/jcp.28869
Abstrakt: In continuation of the investigation of osteogenic potential of solvent fractions of ethanolic extract of Cissus quadrangularis (CQ), an ancient medicinal plant, most notably known for its bone-healing properties, to isolate and identify antiosteoporotic compounds. In the current study, we report the effect of hexane fraction (CQ-H) and dichloromethane fraction (CQ-D) of CQ on the differentiation and mineralization of mouse preosteoblast cell line MC3T3-E1 (subclone 4). Growth, viability, and proliferation assays revealed that low concentrations (0.1, 1, and 100 ng/ml) of both solvent fractions were nontoxic, whereas higher concentrations were toxic to the cells. Differentiation and mineralization of MC3T3-E1 with nontoxic concentrations of CQ-D and CQ-H revealed that CQ-D delayed the mineralization of MC3T3-E1 cells. However, early and enhanced mineralization was observed in cultures treated with nontoxic concentrations of CQ-H, as indicated by Von Kossa staining and expression profile of osteoblast marker genes such as osterix, Runx2, alkaline phosphatase (ALP), collagen (Col1a1), integrin-related bone sialoprotein (IBSP), osteopontin (OPN), and osteocalcin (OCN). These findings suggest CQ-H as the most efficacious solvent fraction for further investigation to isolate and identify the active compounds in CQ-H.
(© 2019 Wiley Periodicals, Inc.)
Databáze: MEDLINE
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