Genomic characterization of multidrug-resistant ESBL-producing Klebsiella pneumoniae isolated from a Ghanaian teaching hospital.
| Autor: | Agyepong N; Antimicrobial Research Unit, College of Health Sciences, University of KwaZulu-Natal, Durban, South Africa. Electronic address: agyanicho33@yahoo.com., Govinden U; Antimicrobial Research Unit, College of Health Sciences, University of KwaZulu-Natal, Durban, South Africa., Owusu-Ofori A; School of Medical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana., Amoako DG; Antimicrobial Research Unit, College of Health Sciences, University of KwaZulu-Natal, Durban, South Africa; Infection Genomics and Applied Bioinformatics Division, Antimicrobial Research Unit, College of Health Sciences, University of KwaZulu-Natal, Durban, South Africa., Allam M; Sequencing Core Facility, National Institute for Communicable Disease, National Health Laboratory Service, South Africa., Janice J; Norwegian National Advisory Unit on Detection of Antimicrobial Resistance, Department of Microbiology and Infection Control, University Hospital of North Norway, Tromso, Norway., Pedersen T; Norwegian National Advisory Unit on Detection of Antimicrobial Resistance, Department of Microbiology and Infection Control, University Hospital of North Norway, Tromso, Norway., Sundsfjord A; Norwegian National Advisory Unit on Detection of Antimicrobial Resistance, Department of Microbiology and Infection Control, University Hospital of North Norway, Tromso, Norway; Department of Medical Biology, Faculty of Health Sciences, UiT - The Arctic University of Norway, Norway., Essack S; Antimicrobial Research Unit, College of Health Sciences, University of KwaZulu-Natal, Durban, South Africa. |
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| Jazyk: | angličtina |
| Zdroj: | International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases [Int J Infect Dis] 2019 Aug; Vol. 85, pp. 117-123. Date of Electronic Publication: 2019 May 23. |
| DOI: | 10.1016/j.ijid.2019.05.025 |
| Abstrakt: | Objectives: This study delineated the clonal lineages, antibiotic resistome and plasmid replicon types in multidrug-resistant K. pneumoniae isolates from a teaching hospital in Ghana. Methods: Identification and antibiotic susceptibility testing were done using the MALDI-TOF MS and Vitek-2 automated system. Genomic DNA extraction was carried out using the NucliSens easyMAG® (BioMérieux) kits and the DNA was subjected to whole genome sequencing (WGS) using the Illumina MiSeq platform. Results: Of the 200 isolates obtained, 37 were identified as K. pneumoniae of which 9 were resistant to all second and third-generation cephalosporins. These 9 isolates selected for further genomic analysis were characterized by the presence of 8 diverse sequence types (STs), capsular polysaccharide serotypes (K types and wzi allelic types) and multiple genes encoding resistance to β-lactams (bla Conclusions: The varied clonal clusters and resistome identified in the multidrug-resistant K. pneumoniae isolates is a major threat to the management of infections in Ghana. The molecular characterization of antibiotic resistance is thus imperative to inform strategies for containment. (Copyright © 2019 The Authors. Published by Elsevier Ltd.. All rights reserved.) |
| Databáze: | MEDLINE |
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