Genomic comparison of Newcastle disease viruses isolated in Nigeria between 2002 and 2015 reveals circulation of highly diverse genotypes and spillover into wild birds.

Autor: Welch CN; Exotic and Emerging Avian Viral Disease Research Unit, Southeast Poultry Research Laboratory, U.S. National Poultry Research Lab, United States Department of Agriculture, 934 College Station Road, Athens, GA, 30605, USA.; Daniel B. Warnell School of Forestry and Natural Resources, University of Georgia, 180 East Green Street, Athens, GA, 30602, USA., Shittu I; National Veterinary Research Institute, PMB 01 Vom, Plateau State, Nigeria., Abolnik C; Department of Production Animal Studies, Faculty of Veterinary Science, University of Pretoria, P/Bag X04, Onderstepoort, 0110, South Africa., Solomon P; National Veterinary Research Institute, PMB 01 Vom, Plateau State, Nigeria.; Department of Production Animal Studies, Faculty of Veterinary Science, University of Pretoria, P/Bag X04, Onderstepoort, 0110, South Africa., Dimitrov KM; Exotic and Emerging Avian Viral Disease Research Unit, Southeast Poultry Research Laboratory, U.S. National Poultry Research Lab, United States Department of Agriculture, 934 College Station Road, Athens, GA, 30605, USA., Taylor TL; Exotic and Emerging Avian Viral Disease Research Unit, Southeast Poultry Research Laboratory, U.S. National Poultry Research Lab, United States Department of Agriculture, 934 College Station Road, Athens, GA, 30605, USA., Williams-Coplin D; Exotic and Emerging Avian Viral Disease Research Unit, Southeast Poultry Research Laboratory, U.S. National Poultry Research Lab, United States Department of Agriculture, 934 College Station Road, Athens, GA, 30605, USA., Goraichuk IV; Exotic and Emerging Avian Viral Disease Research Unit, Southeast Poultry Research Laboratory, U.S. National Poultry Research Lab, United States Department of Agriculture, 934 College Station Road, Athens, GA, 30605, USA., Meseko CA; National Veterinary Research Institute, PMB 01 Vom, Plateau State, Nigeria., Ibu JO; Department of Veterinary Pathology and Microbiology, University of Agriculture, Makurdi, Benue, Nigeria., Gado DA; National Veterinary Research Institute, PMB 01 Vom, Plateau State, Nigeria.; Department of Production Animal Studies, Faculty of Veterinary Science, University of Pretoria, P/Bag X04, Onderstepoort, 0110, South Africa., Joannis TM; National Veterinary Research Institute, PMB 01 Vom, Plateau State, Nigeria., Afonso CL; Exotic and Emerging Avian Viral Disease Research Unit, Southeast Poultry Research Laboratory, U.S. National Poultry Research Lab, United States Department of Agriculture, 934 College Station Road, Athens, GA, 30605, USA. claudio.afonso@ars.usda.gov.
Jazyk: angličtina
Zdroj: Archives of virology [Arch Virol] 2019 Aug; Vol. 164 (8), pp. 2031-2047. Date of Electronic Publication: 2019 May 23.
DOI: 10.1007/s00705-019-04288-9
Abstrakt: Newcastle disease virus (NDV) has a wide avian host range and a high degree of genetic variability, and virulent strains cause Newcastle disease (ND), a worldwide concern for poultry health. Although NDV has been studied in Nigeria, genetic information about the viruses involved in the endemicity of the disease and the transmission that likely occurs at the poultry-wildlife interface is still largely incomplete. Next-generation and Sanger sequencing was performed to provide complete (n = 73) and partial genomic sequence data (n = 38) for NDV isolates collected from domestic and wild birds in Nigeria during 2002-2015, including the first complete genome sequences of genotype IV and subgenotype VIh from the African continent. Phylogenetic analysis revealed that viruses of seven different genotypes circulated in that period, demonstrating high genetic diversity of NDV for a single country. In addition, a high degree of similarity between NDV isolates from domestic and wild birds was observed, suggesting that spillovers had occurred, including to three species that had not previously been shown to be susceptible to NDV infection. Furthermore, the first spillover of a mesogenic Komarov vaccine virus is documented, suggesting a previous spillover and evolution of this virus. The similarities between viruses from poultry and multiple bird species and the lack of evidence for host adaptation in codon usage suggest that transmission of NDV between poultry and non-poultry birds occurred recently. This is especially significant when considering that some viruses were isolated from species of conservation concern. The high diversity of NDV observed in both domestic and wild birds in Nigeria emphasizes the need for active surveillance and epidemiology of NDV in all bird species.
Databáze: MEDLINE
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