| Autor: |
Liu AY, Mack PW, Champion CI, Robinson RR |
| Jazyk: |
angličtina |
| Zdroj: |
Gene [Gene] 1987; Vol. 54 (1), pp. 33-40. |
| DOI: |
10.1016/0378-1119(87)90344-1 |
| Abstrakt: |
A chimeric mouse variable::human constant immunoglobulin heavy-chain gene was expressed in transfected mouse Sp2/0 cells. The chimeric immunoglobulin genes were integrated in tandem in the genome of stably transformed cells. These integrated gene copies were amplified by selection with a second drug marker. The gene amplification led to an increase in the expression of chimeric heavy-chain protein. The level of gene expression appears to be related to the site of integration; a few gene copies in one transfectant can yield as much heavy-chain protein as many copies in a second transfectant. In addition, we found that an adventitious oligo(C) sequence, introduced by our method of gene construction at a site located 8 nt residues downstream from a splice acceptor, can apparently direct splicing towards a cryptic splice acceptor downstream from the oligo(C). |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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