[Molecular identification of Aspergillus fumigatus isolated from patients with invasive aspergillosis].

Autor: Béjar V; Instituto de Medicina Tropical 'Daniel A. Carrión'. Lima, Perú.; Departamento Académico de Microbiología Médica, Universidad Nacional Mayor de San Marcos. Lima, Perú., Villanueva F; Departamento Académico de Microbiología Médica, Universidad Nacional Mayor de San Marcos. Lima, Perú.; Instituto Nacional de Enfermedades Neoplásicas. Lima, Perú., León SR; Instituto de Medicina Tropical 'Daniel A. Carrión'. Lima, Perú.; Universidad Privada San Juan Bautista. Lima, Perú., Guevara-Granados JM; Instituto de Medicina Tropical 'Daniel A. Carrión'. Lima, Perú.; Departamento Académico de Microbiología Médica, Universidad Nacional Mayor de San Marcos. Lima, Perú., Uribe A; Hospital Nacional Dos de Mayo. Lima, Perú., Vergaray G; Instituto de Investigación de Ciencias Biológicas 'Antonio Raimondi'. Lima, Perú., Cuadra A; Instituto de Medicina Tropical 'Daniel A. Carrión'. Lima, Perú.; Departamento Académico de Microbiología Médica, Universidad Nacional Mayor de San Marcos. Lima, Perú., Sabogal I; Instituto de Medicina Tropical 'Daniel A. Carrión'. Lima, Perú.; Departamento Académico de Microbiología Médica, Universidad Nacional Mayor de San Marcos. Lima, Perú.
Jazyk: Spanish; Castilian
Zdroj: Revista peruana de medicina experimental y salud publica [Rev Peru Med Exp Salud Publica] 2019 Jan-Mar; Vol. 36 (1), pp. 81-86. Date of Electronic Publication: 2019 May 13.
DOI: 10.17843/rpmesp.2019.361.3403
Abstrakt: The objective of the study was to identify molecularly-isolated strains of Aspergillus from patients with invasive aspergillosis (IA); these strains were primarily typed as Aspergillus fumigatus sensu lato by conventional phenotypic methods. We worked with 20 strains from the mycology section of the Institute of Tropical Medicine "Daniel A. Carrión." To obtain the fungal DNA, thermal shock, enzymatic treatment, and silica gel column techniques were used; and it was stored at -20°C to preserve it. The real-time polymerase chain reaction (qPCR) procedure included fluorochrome-labeled primers, which amplified the specific sequences of A. fumigatus. Fluorescence was measured with the thermocycler at the end of the hybridization phase of each cycle. It was molecularly-identified that only 50% of the strains studied belong to the species Aspergillus fumigatus sensu stricto.
Databáze: MEDLINE
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