Engram-specific transcriptome profiling of contextual memory consolidation.

Autor:	Rao-Ruiz P; Department of Psychiatry, Erasmus MC University Medical Center, Rotterdam, 3015 GD, The Netherlands.; Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Amsterdam Neuroscience, Vrije Universiteit Amsterdam, Amsterdam, 1081 HV, The Netherlands., Couey JJ; Department of Psychiatry, Erasmus MC University Medical Center, Rotterdam, 3015 GD, The Netherlands., Marcelo IM; Department of Psychiatry, Erasmus MC University Medical Center, Rotterdam, 3015 GD, The Netherlands.; Champalimaud Neuroscience Programme, Champalimaud Centre for the Unknown, Lisbon, 1400-038, Portugal., Bouwkamp CG; Department of Psychiatry, Erasmus MC University Medical Center, Rotterdam, 3015 GD, The Netherlands., Slump DE; Department of Psychiatry, Erasmus MC University Medical Center, Rotterdam, 3015 GD, The Netherlands., Matos MR; Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Amsterdam Neuroscience, Vrije Universiteit Amsterdam, Amsterdam, 1081 HV, The Netherlands., van der Loo RJ; Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Amsterdam Neuroscience, Vrije Universiteit Amsterdam, Amsterdam, 1081 HV, The Netherlands., Martins GJ; Champalimaud Neuroscience Programme, Champalimaud Centre for the Unknown, Lisbon, 1400-038, Portugal.; Department of Neuroscience, Zuckerman Mind Brain Behavior Institute, Columbia University, New York, 10027, NY, USA., van den Hout M; Center for Biomics, Erasmus MC University Medical Center, Rotterdam, 3015 GD, The Netherlands., van IJcken WF; Center for Biomics, Erasmus MC University Medical Center, Rotterdam, 3015 GD, The Netherlands., Costa RM; Champalimaud Neuroscience Programme, Champalimaud Centre for the Unknown, Lisbon, 1400-038, Portugal.; Department of Neuroscience, Zuckerman Mind Brain Behavior Institute, Columbia University, New York, 10027, NY, USA., van den Oever MC; Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Amsterdam Neuroscience, Vrije Universiteit Amsterdam, Amsterdam, 1081 HV, The Netherlands. michel.vanden.oever@vu.nl., Kushner SA; Department of Psychiatry, Erasmus MC University Medical Center, Rotterdam, 3015 GD, The Netherlands. s.kushner@erasmusmc.nl.
Jazyk:	angličtina
Zdroj:	Nature communications [Nat Commun] 2019 May 20; Vol. 10 (1), pp. 2232. Date of Electronic Publication: 2019 May 20.
DOI:	10.1038/s41467-019-09960-x
Abstrakt:	Sparse populations of neurons in the dentate gyrus (DG) of the hippocampus are causally implicated in the encoding of contextual fear memories. However, engram-specific molecular mechanisms underlying memory consolidation remain largely unknown. Here we perform unbiased RNA sequencing of DG engram neurons 24 h after contextual fear conditioning to identify transcriptome changes specific to memory consolidation. DG engram neurons exhibit a highly distinct pattern of gene expression, in which CREB-dependent transcription features prominently (P = 6.2 × 10 -13 ), including Atf3 (P = 2.4 × 10 -41 ), Penk (P = 1.3 × 10 -15 ), and Kcnq3 (P = 3.1 × 10 -12 ). Moreover, we validate the functional relevance of the RNAseq findings by establishing the causal requirement of intact CREB function specifically within the DG engram during memory consolidation, and identify a novel group of CREB target genes involved in the encoding of long-term memory.
Databáze:	MEDLINE
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