Enhanced antibacterial activity of hemocyanin purified from Portunus pelagicus hemolymph combined with silver nanoparticles - Intracellular uptake and mode of action.
Autor: | Ishwarya R; Crustacean Molecular Biology and Genomics Division, Biomaterials and Biotechnology in Animal Health Lab, Department of Animal Health and Management, Alagappa University, Science Block, 6th floor, Burma Colony, Karaikudi, 630 004, Tamil Nadu, India., Vaseeharan B; Crustacean Molecular Biology and Genomics Division, Biomaterials and Biotechnology in Animal Health Lab, Department of Animal Health and Management, Alagappa University, Science Block, 6th floor, Burma Colony, Karaikudi, 630 004, Tamil Nadu, India. Electronic address: vaseeharanb@gmail.com., Shanthini S; Centre for Animal Science Research and Extension Services, Foundation for Innovative Research in Science and Technology, Kelavannanvilai, NGO Colony Road, Nagercoil, Tamil Nadu, India., Govindarajan M; Unit of Vector Control, Phytochemistry and Nanotechnology, Department of Zoology, Annamalai University, Annamalainagar, Tamil Nadu, 608 002, India; Department of Zoology, Government College for Women, Kumbakonam, 612 001, Tamil Nadu, India., Alharbi NS; Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia. Electronic address: nalharbi1@ksu.edu.sa., Kadaikunnan S; Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia., Khaled JM; Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia., Al-Anbr MN; Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia. |
---|---|
Jazyk: | angličtina |
Zdroj: | Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS) [J Trace Elem Med Biol] 2019 Jul; Vol. 54, pp. 8-20. Date of Electronic Publication: 2019 Mar 11. |
DOI: | 10.1016/j.jtemb.2019.03.005 |
Abstrakt: | Recently, biogenic nanoparticles have been considered promising candidates for manufacturing antibacterial nanodrugs. Here, we synthesized AgNPs using the crab-borne antibacterial agent hemocyanin and assessed the antibacterial action against several pathogenic bacteria. In this study, the crustacean immune protein hemocyanin (Pp-Hc, 78 kDa) purified from Portunus pelagicus hemolymph was used to fabricate silver nanoparticles. Characterization of hemocyanin-fabricated AgNPs (Pp-Hc AgNPs) were achieved using ultraviolet-visible spectrophotometer, X-ray powder diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), high-resolution-transmission electron microscopy (HR-TEM), and energy-dispersive X-ray spectroscopy. The antibacterial efficacy of AgNO (Copyright © 2019 Elsevier GmbH. All rights reserved.) |
Databáze: | MEDLINE |
Externí odkaz: |