Comparison of aqueous and enzymatic extraction combination with sequential filtration for the profiling of selected trace elements in medicinal plants from Kenya.

Autor: Mogwasi R; Department of Chemistry, Kisii University, P.O. Box 408-40200, Kisii, Kenya; Department of Chemistry, University of Nairobi, P.O. Box 30197-00100, Nairobi, Kenya. Electronic address: mogwasirichard@gmail.com., Kariuki DK; Department of Chemistry, University of Nairobi, P.O. Box 30197-00100, Nairobi, Kenya., Getenga MZ; Department of Chemistry, Chuka University, P.O. Box 109-60400, Chuka, Kenya., Nischwitz V; Central Institute for Engineering, Electronics and Analytics, Analytics (ZEA-3), Forschungszentrum Juelich, 52425, Juelich, Germany.
Jazyk: angličtina
Zdroj: Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS) [J Trace Elem Med Biol] 2019 Jul; Vol. 54, pp. 1-7. Date of Electronic Publication: 2019 Mar 09.
DOI: 10.1016/j.jtemb.2019.03.003
Abstrakt: This work presents results for the profiling of eight essential elements (Co, Cu, Ni, V, Mo, Mn, Zn and Cr) in aqueous and enzymatic extracts of eight anti-diabetic medicinal plants, used by Traditional Medicine Practitioners from Nyamira County, Kenya determined by ICP-MS. The plants used in the study were Solanum indicum, Plectranthus barbatus, Ultrica dioica, Bidens pilosa, Solanum mauense, Clerodendrum myricoides, Carissa edulis and Aloe vera. A sequential filtration procedure was applied to fractionate the elemental contents of the obtained aqueous extracts into molecular size fractions. The results indicate that the low molecular size species (<3 kDa) were predominant for Mo, Zn, Ni, Co, Mn and Cu, while the moderately large species (10 kDa-0.45 μm) of V were predominant in most of the medicinal plant extracts. In addition enzymatic extraction was compared to aqueous extraction to study the effect of the gastric and intestinal conditions on the release of selected elements from the plants. The amount of the elements extracted by the gastric phase enzymes was higher than the amount extracted by the intestinal phase enzymes. In general, the determined elemental amounts of enzymatic extractions were higher than those of corresponding water extractions for 70% of the elements studied.
(Published by Elsevier GmbH.)
Databáze: MEDLINE
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