Hyperglycemic condition influence on mineral trioxide aggregate biocompatibility and biomineralization.

Autor: de Azevedo Queiroz ÍO; Department of Endodontics, Araçatuba Dental School, UNESP, Araçatuba, Brazil., Gonçalves LO; Department of Endodontics, Araçatuba Dental School, UNESP, Araçatuba, Brazil., Takamiya AS; Department of Dental Materials and Prosthodontics, Araçatuba Dental School, UNESP, Araçatuba, Brazil., Rezende GC; Department of Endodontics, Araçatuba Dental School, UNESP, Araçatuba, Brazil., Machado T; Department of Oral and Maxillofacial Surgery and Integrated Clinic, Araçatuba Dental School, UNESP, Araçatuba, Brazil., Siveiri-Araujo G; Department of Endodontics, Araçatuba Dental School, UNESP, Araçatuba, Brazil., Ervolino E; Department of Basic Science, Araçatuba Dental School, UNESP, Araçatuba, Brazil., Oliveira SHP; Department of Basic Science, Araçatuba Dental School, UNESP, Araçatuba, Brazil., Gomes-Filho JE; Department of Endodontics, Araçatuba Dental School, UNESP, Araçatuba, Brazil.
Jazyk: angličtina
Zdroj: Journal of biomedical materials research. Part B, Applied biomaterials [J Biomed Mater Res B Appl Biomater] 2020 Apr; Vol. 108 (3), pp. 663-673. Date of Electronic Publication: 2019 May 20.
DOI: 10.1002/jbm.b.34420
Abstrakt: This study aimed to investigate in vitro and in vivo the influence of hyperglycemic condition on biocompatibility and biomineralization of gray mineral trioxide aggregate (GMTA) and white mineral trioxide aggregate (WMTA). For the in vitro study, fibroblast-like cells L929 were cultured under high or normal glucose concentration to investigate the effects of both MTA's on cell proliferation and inflammatory cytokines production IL-1β, IL-6, and TNF-α. For the in vivo study, polyethylene tubes containing MTA materials and empty tubes were implanted into dorsal connective tissues of Wistar rats previously assigned normal and hyperglycemic. After 7 and 30 days, the tubes with surrounding tissues were removed and subjected to histological, fluorescence and immunohistochemical analyzes of IL-1β, IL-6, and TNF-α. In vitro study showed that, under high glucose condition, GMTA reduced cell proliferation and IL-6 production compared with WMTA. Moreover, in vivo study revealed that hyperglycemic condition did not modify the inflammatory response and cytokines production in the tissue close to both materials. Independently of hyperglycemic status, mineralized areas were observed with both materials, but the fluorescence intensity of WMTA was diminished on 14 days in hyperglycemic animals. It is possible to conclude that GMTA was able to inhibit the proliferation rate and IL-6 production under high glucose concentration in vitro. Furthermore, cytokines production and inflammatory response were not upregulated in hyperglycemic animals; however, a decrease in the calcium deposition was observed in presence of WMTA, suggesting a delay in the mineralization process.
(© 2019 Wiley Periodicals, Inc.)
Databáze: MEDLINE
Externí odkaz: