Assessment of Impact of Various Root Canal Irrigants on the Adherence of the Gelatinase-producing and the Gelatinase-deficient E. faecalis Strains to Dentin.

Autor:	Rana M; Department of Conservative Dentistry and Endodontics, College of Medical Sciences, Bharatpur, Nepal., Jain V; Department of Conservative Dentistry and Endodontics, Chhattisgarh Dental College and Research Institute, Rajnandgaon, Chhattisgarh, India., Kaur P; Department of Conservative and Endodontic, BJS Dental College and Hospital and Research Institute, Ludhiana, Punjab, India., Jalan P; Private Practitioner, Agra, Uttar Pradesh, India., Kapadia JM; Department of Public Health Dentistry, Bhabha College of Dental Sciences, Bhopal, Madhya Pradesh, India, Phone: 7303320242, e-mail: drjunaid.kapadia@gmail.com., Shaikh I; Department of Conservative Dentistry and Endodontics, ACPM Dental College, Dhule, Maharashtra, India.
Jazyk:	angličtina
Zdroj:	The journal of contemporary dental practice [J Contemp Dent Pract] 2019 Jan 01; Vol. 20 (1), pp. 46-50. Date of Electronic Publication: 2019 Jan 01.
Abstrakt:	Aim: Present study was planned to assess the impact of various root canal irrigants on the adhesion of different Enterococcus faecalis ( E. faecalis ) strains to the dentinal surface. Materials and Methods: A total of 80 freshly extracted first and second molars were used in the present study. Preparation of dentin discs was done followed by a random division into four study groups and one control group. Four study groups included; 2.5 % sodium hypochlorite (NaOCl), 2 % chlorhexidine (CHX), 2.5 % NaOCl + saline + 2 percent CHX and 2.5 % NaOCl + 17 % ethylene diamine tetra-acetate (EDTA) + 2.5 % NaOCl group respectively. In the control group (E), sterilized dentin discs were incubated with sterile TSB solution. Division of all the groups into two subgroups were done depending upon the type of strain of E. faecalis used. Incubation of all the specimens was done followed by assessment with XTT assay and measurement of Optical density (OD). All the results were compiled and analyzed by Statistical Package for the Social Sciences (SPSS) software. Results: Among the groups containing gelatinase producing strains; maximum score was exhibited by 2.5 percent sodium hypochlorite solution followed by 2.5 % NaOCl + 17 % Ethylene Diamine tetra-acetate (EDTA) + 2.5 % NaOCl group (group D). On comparing the OD values among various study groups incubated with Gelatinase producing strain, significant results were obtained. Gelatinase-producing E. faecalis showed a significantly higher amount of adherence to dentin, in comparison to the gelatinase-deficient E. faecalis strains. Conclusion: Lesser quantity of bacteria is recovered from specimens in whom CHX was added to the irrigation protocol. Clinical Significance: Production of gelatinase by E. faecalis might lead to an increase in adhesiveness of E. faecalis to the dentin.
Databáze:	MEDLINE
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