Acoustic droplet vaporization-mediated dissolved oxygen scavenging in blood-mimicking fluids, plasma, and blood.

Autor: Mercado-Shekhar KP; Department of Internal Medicine, University of Cincinnati, Cincinnati, OH, USA., Su H; Department of Internal Medicine, University of Cincinnati, Cincinnati, OH, USA., Kalaikadal DS; Department of Mechanical and Materials Engineering, University of Cincinnati, Cincinnati, OH, USA., Lorenz JN; Department of Pharmacology and Systems Physiology, University of Cincinnati, Cincinnati, OH, USA., Manglik RM; Department of Mechanical and Materials Engineering, University of Cincinnati, Cincinnati, OH, USA., Holland CK; Department of Internal Medicine, University of Cincinnati, Cincinnati, OH, USA; Department of Biomedical Engineering, University of Cincinnati, Cincinnati, OH, USA., Redington AN; Division of Cardiology, The Heart Institute, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA., Haworth KJ; Department of Internal Medicine, University of Cincinnati, Cincinnati, OH, USA; Department of Biomedical Engineering, University of Cincinnati, Cincinnati, OH, USA; Division of Cardiology, The Heart Institute, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA. Electronic address: kevin.haworth@uc.edu.
Jazyk: angličtina
Zdroj: Ultrasonics sonochemistry [Ultrason Sonochem] 2019 Sep; Vol. 56, pp. 114-124. Date of Electronic Publication: 2019 Mar 28.
DOI: 10.1016/j.ultsonch.2019.03.029
Abstrakt: Acoustic droplet vaporization (ADV) has been shown to reduce the partial pressure of oxygen (PO 2 ) in a fluid. The goals of this study were three-fold: 1) to determine the ADV pressure amplitude threshold in fluids that had physiologically relevant values for surface tension, protein concentration, and viscosity; 2) to assess whether these parameters and fluid mixing affect ADV-mediated PO 2 reduction; and 3) to assess the feasibility of ADV-mediated PO 2 reduction in plasma and whole blood. In vitro ADV experiments were conducted using perfluoropentane droplets (number density: 5 × 10 6  ± 0.2 × 10 6 /mL) dispersed in fluids (saline, polyvinylpyrrolidone solutions, porcine plasma, or porcine whole blood) that had a physiological range of surface tensions (62-68 mN/m), protein concentrations (0 and 68.7 mg/mL), and viscosities (0.7-4 cP). Droplets were exposed to pulsed ultrasound (5 MHz, 4.25 MPa peak negative pressure) while passing through a 37 °C flow system with inline PO 2 sensors. In select experiments, the fluid also passed through mixing channels after ultrasound exposure. Our results revealed that the ADV pressure thresholds were the same for all fluids. Surface tension and protein concentration had no effect on PO 2 reduction. Increasing viscosity attenuated PO 2 reduction. However, the attenuated effect was absent after fluid mixing. Furthermore, ADV-mediated PO 2 reduction in whole blood (30.8 ± 3.2 mmHg) was less than that in a polyvinylpyrrolidone solution (40.2 ± 2.1 mmHg) with equal viscosity. These findings should be considered when planning clinical studies of ADV-mediated PO 2 reduction and other biomedical applications of ADV.
(Copyright © 2019 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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