Oxidative DNA Damage Is Increased in Living Kidney Donors.

Autor: Karahan M; Department of General Surgery, Kartal Training Hospital, Istanbul, Turkey., Yildirim M; Department of Transplantation, Kartal Training Hospital, Istanbul, Turkey., Kucuk HF; Department of Transplantation, Kartal Training Hospital, Istanbul, Turkey., Turunc V; Bahcesehir University, Department of General Surgery, Istanbul, Turkey., Demir H; Department of Biochemistry, Yuzuncu Yil University, Van, Turkey., Salturk C; Department of Chest Diseases, Yeniyuzyil University, Istanbul, Turkey., Yavuz A; Department of Nephrology, Tekirdag State Hospital, Tekirdag, Turkey., Demir T; Department of Transplantation, Kartal Training Hospital, Istanbul, Turkey., Ari E; Bahcesehir University, Department of Nephrology, Istanbul, Turkey. Electronic address: elifaribakir@gmail.com.
Jazyk: angličtina
Zdroj: Transplantation proceedings [Transplant Proc] 2019 May; Vol. 51 (4), pp. 1049-1053. Date of Electronic Publication: 2019 Feb 21.
DOI: 10.1016/j.transproceed.2019.02.011
Abstrakt: Background: Long-term consequences of donor nephrectomy might be reduced kidney function, increased risk for cardiovascular disease, and impaired quality of life. The purpose of the current cross-sectional study was to evaluate the relationship between clinical, laboratory, and donation-specific outcomes of living kidney donors and systemic oxidative DNA damage.
Methods: We conducted a cross-sectional study and assessed retrospectively pre- and postdonation data from 60 donors who donated between 2010 and 2015. Plasma malondialdehyde levels and 8-hydroxy-2'-deoxyguanosine/deoxyguanosine ratio (8-OHdG/dG ratio) were determined as oxidative stress markers. Catalase, carbonic anhydrase, and paraoxonase (PON) activities were measured as antioxidants.
Results: Approximately 3 years after donation, the hypertensive donor ratio was 12%, and 11% of the donors had glomerular filtration rate <60 mL/min/1.73 m 2 . Mean serum urea (P = .001) and serum creatinine levels (P = .001) were increased; creatinine clearance level (126.2 ± 35.5 vs 94.6 ± 26.8, P = .001) was decreased in the postdonation period. There was a significant positive correlation between predonation serum urea and 8-0HdG/dG ratio (r = 0.338, P = .016) and predonation serum creatinine and 8-0HdG/dG ratio (r = 0.442, P = .001), while there was a significant negative correlation between serum creatinine and PON activity (r = -0.545, P < .001).
Conclusion: Our data have demonstrated that kidney donors exhibit increased oxidative DNA damage and decreased antioxidant activity. We propose that predonation serum creatinine is positively correlated with 8-0HdG/dG ratio and negatively correlated with antioxidant PON activity. This is the first study to demonstrate that plasma oxidative DNA damage increases in healthy kidney donors.
(Copyright © 2019 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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