Investigation of Anti-HLA Antibodies of Highly Sensitized Patients by Single Antigen Bead and C1q Tests.

Autor: Ayna TK; Medical Biology and Genetics Department, Faculty of Medicine, Izmir Katip Celebi University, Izmir, Turkey; Tissue Typing Laboratory, Tepecik Education and Research Hospital, Izmir, Turkey. Electronic address: tulayayna@gmail.com., Koçyİğİt AÖ; Medical Biology and Genetics Department, Faculty of Medicine, Izmir Katip Celebi University, Izmir, Turkey; Tissue Typing Laboratory, Tepecik Education and Research Hospital, Izmir, Turkey., Soypaçaci Z; Nephrology, Izmir Katip Celebi University Atatürk Education and Research Hospital, Izmir, Turkey., Tuğmen C; General Surgery, Tepecik Education and Research Hospital, Izmir, Turkey., Pirim I; Medical Biology and Genetics Department, Faculty of Medicine, Izmir Katip Celebi University, Izmir, Turkey; Tissue Typing Laboratory, Tepecik Education and Research Hospital, Izmir, Turkey.
Jazyk: angličtina
Zdroj: Transplantation proceedings [Transplant Proc] 2019 May; Vol. 51 (4), pp. 1024-1026. Date of Electronic Publication: 2019 Feb 08.
DOI: 10.1016/j.transproceed.2019.01.086
Abstrakt: Purpose: The single antigen bead (SAB) test contributes to conventional cellular and solid phase crossmatch tests in renal transplantation. However, the determination of anti-HLA antibodies of the patients may not reflect the pathologic features of these antibodies. Highly sensitized patients produce antibodies against a number of HLAs; therefore, their transplantation chance decreases. In this study, we aimed to evaluate SAB and C1q test results of highly sensitized patients.
Method: In this study, 33 end-stage renal failure patients with >80% panel reactive antibody were included. Of the patients, 58% (n = 19) were female, and 42% (n = 14) were male. The mean age was 46.2 ± 12.4. All of the serum samples were inactivated by heat before use. SAB and C1q tests were performed according to the manufacturer's instructions.
Results: We obtained statistically significant results between the positive bead counts and raw mean fluorescence intensity (MFI) values of 2 tests (P < .01 for class I and II). There was a statistically significant difference between the 2 tests in terms HLA-A, -C, -DR, and -DP MFI values, whereas HLA-B and -DQ MFI values were similar for the 2 tests.
Conclusion: The difference of raw MFI values between the 2 tests may be due to the fact that the C1q test detects only IgG1 and IgG3 antibodies, whereas the SAB test can detect all IgG subtypes. We considered that anti-HLA-B and -DQ antibodies have high complement-fixing features; these antibodies should be investigated selectively due to the similarity of anti-HLA-B and -DQ antibody MFI values in the 2 tests.
(Copyright © 2019 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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