Catechin attenuates TNF-α induced inflammatory response via AMPK-SIRT1 pathway in 3T3-L1 adipocytes.

Autor: Cheng AW; Institute of Agro-food Science and Technology, Shandong Academy of Agricultural Sciences, Jinan, China.; Key Laboratory of Novel Food Resources Processing, Ministry of Agriculture, Jinan, China.; Key Laboratory of Agro-Products Processing Technology of Shandong Province, Jinan, China., Tan X; Institute of Agro-food Science and Technology, Shandong Academy of Agricultural Sciences, Jinan, China.; Key Laboratory of Novel Food Resources Processing, Ministry of Agriculture, Jinan, China.; Key Laboratory of Agro-Products Processing Technology of Shandong Province, Jinan, China.; College of Food Science and Engineering, Jilin Agricultural University, Changchun, China., Sun JY; Institute of Agro-food Science and Technology, Shandong Academy of Agricultural Sciences, Jinan, China.; Key Laboratory of Novel Food Resources Processing, Ministry of Agriculture, Jinan, China.; Key Laboratory of Agro-Products Processing Technology of Shandong Province, Jinan, China., Gu CM; College of Food Science and Engineering, Jilin Agricultural University, Changchun, China., Liu C; Institute of Agro-food Science and Technology, Shandong Academy of Agricultural Sciences, Jinan, China.; Key Laboratory of Novel Food Resources Processing, Ministry of Agriculture, Jinan, China.; Key Laboratory of Agro-Products Processing Technology of Shandong Province, Jinan, China., Guo X; Institute of Agro-food Science and Technology, Shandong Academy of Agricultural Sciences, Jinan, China.; Key Laboratory of Novel Food Resources Processing, Ministry of Agriculture, Jinan, China.; Key Laboratory of Agro-Products Processing Technology of Shandong Province, Jinan, China.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2019 May 17; Vol. 14 (5), pp. e0217090. Date of Electronic Publication: 2019 May 17 (Print Publication: 2019).
DOI: 10.1371/journal.pone.0217090
Abstrakt: Chronic inflammation is a fundamental symptom of many diseases. Catechin possesses anti-oxidant and anti-inflammatory properties. However, the mechanism of catechin to prevent inflammation in 3T3-L1 adipocytes caused by TNF-α remains unknown. Therefore, the effects of catechin on the gene expression of cytokines and the activation of cell signals in TNF-α induced 3T3-L1 adipocytes were investigated. The effects of catechin on adipogenesis and cell viability were detected by Oil Red O staining and CCK-8 assay, respectively. The genes expression of cytokines was determined by real-time RT-PCR. The expression of NF-κB, AMPK, FOXO3a and SIRT1 on translation level was determined by western blotting analysis. The results demonstrated that catechin significantly enhanced adipogenesis and cell viability. catechin inhibited the gene expression of pro-inflammatory cytokines including IL-1α, IL-1β, IL-6, IL-12p35, and inflammatory enzymes including iNOS and COX-2, but enhanced the gene expression of anti-inflammatory cytokines including IL-4 and IL-10. Catechin also inhibited the activation of NF-κB, AMPK, FOXO3a and SIRT1, but increased the phosphorylation level of the above factors. All these results indicated that as a potential therapeutic strategy catechin has the ability of attenuating inflammatory response triggered by TNF-α through signaling cascades involved in inflammation and cytokines.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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