Intravital imaging allows real-time characterization of tissue resident eosinophils.

Autor: Chojnacki A; 1Department of Physiology and Pharmacology, Snyder Institute for Chronic Diseases, Cumming School of Medicine, University of Calgary, Calgary, AB Canada., Wojcik K; 1Department of Physiology and Pharmacology, Snyder Institute for Chronic Diseases, Cumming School of Medicine, University of Calgary, Calgary, AB Canada., Petri B; 2Department of Microbiology, Immunology and Infectious Diseases, Snyder Institute for Chronic Diseases, Cumming School of Medicine, University of Calgary, Calgary, AB Canada., Aulakh G; 1Department of Physiology and Pharmacology, Snyder Institute for Chronic Diseases, Cumming School of Medicine, University of Calgary, Calgary, AB Canada.; 3Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK Canada., Jacobsen EA; 4Division of Allergy and Clinical Immunology, Mayo Clinic Arizona, Scottsdale, AZ USA., LeSuer WE; 4Division of Allergy and Clinical Immunology, Mayo Clinic Arizona, Scottsdale, AZ USA., Colarusso P; 1Department of Physiology and Pharmacology, Snyder Institute for Chronic Diseases, Cumming School of Medicine, University of Calgary, Calgary, AB Canada., Patel KD; 1Department of Physiology and Pharmacology, Snyder Institute for Chronic Diseases, Cumming School of Medicine, University of Calgary, Calgary, AB Canada.; 5Department of Biochemistry and Molecular Biology, Snyder Institute for Chronic Diseases, Cumming School of Medicine, University of Calgary, Calgary, AB Canada.
Jazyk: angličtina
Zdroj: Communications biology [Commun Biol] 2019 May 13; Vol. 2, pp. 181. Date of Electronic Publication: 2019 May 13 (Print Publication: 2019).
DOI: 10.1038/s42003-019-0425-3
Abstrakt: Eosinophils are core components of the immune system, yet tools are lacking to directly observe eosinophils in action in vivo. To better understand the role of tissue resident eosinophils, we used eosinophil-specific CRE (eoCRE) mice to create GFP and tdTomato reporters. We then employed intravital microscopy to examine the dynamic behaviour of eosinophils in the healthy GI tract, mesentery, liver, lymph node, skin and lung. Given the role of eosinophils in allergic airway diseases, we also examined eosinophils in the lung following ovalbumin sensitization and challenge. We were able to monitor and quantify eosinophilic behaviours including patrolling, crawling, clustering, tissue distribution and interactions with other leukocytes. Thus, these reporter mice allow eosinophils to be examined in real-time in living animals, paving the way to further understanding the roles eosinophils play in both health and disease.
Competing Interests: The authors declare no competing interests.
Databáze: MEDLINE
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