Serological and molecular detection of Mycoplasma pneumoniae in children with community-acquired lower respiratory tract infections.

Autor: Kumar S; Department of Microbiology, Maulana Azad Medical College, New Delhi 110002, India. Electronic address: kumarsurinderdr@yahoo.com., Garg IB; Department of Microbiology, Maulana Azad Medical College, New Delhi 110002, India. Electronic address: indugarg47@gmail.com., Sethi GR; Department of Pediatrics, Maulana Azad Medical College, New Delhi 110002, India. Electronic address: grsethi56@gmail.com.
Jazyk: angličtina
Zdroj: Diagnostic microbiology and infectious disease [Diagn Microbiol Infect Dis] 2019 Sep; Vol. 95 (1), pp. 5-9. Date of Electronic Publication: 2019 Mar 27.
DOI: 10.1016/j.diagmicrobio.2019.03.010
Abstrakt: This study was designed to evaluate the incidence of Mycoplasma pneumoniae infection in children with community-acquired lower respiratory tract infections (LRTIs). A total of 245 patients 6 months to 12 years of age were investigated for M. pneumoniae employing serological tests, polymerase chain reaction (PCR), nested PCR, and reverse transcription PCR (RT-PCR) on throat swab samples. Forty five (59.2%) children <5 years and 31 (40.7%) children ≥5 years age group were positive for M. pneumoniae infection, and this difference was statistically significant (P ≤ 0.01).Clinical and radiological findings across M.pneumoniae-positive and -negative cases were comparable. Serology, PCR, nested PCR, and RT-PCR together detected M. pneumoniae infection in 76 (31%) patients. Sensitivity, specificity, and positive and negative predictive values of PCR were 16.18%, 95.48%, 57.89%, and 74.78%, respectively, and those of serology were 57.89%, 74.78%, 16.18%, and 95.48%, respectively. Serological and molecular detection in combination is useful for rapid and reliable diagnosis of M. pneumoniae infections in children with LRTIs.
(Copyright © 2019 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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