Synthesis of Polyhydroxyalkanoates by Hydrogen-Oxidizing Bacteria in a Pilot Production Process.

Autor:	Volova T; Siberian Federal University , 79 Svobodnyi Av. , Krasnoyarsk 660041 , Russia.; Institute of Biophysics SB RAS , Federal Research Center 'Krasnoyarsk Science Center SB RAS', 50/50 Akademgorodok , Krasnoyarsk 660036 , Russia., Kiselev E; Siberian Federal University , 79 Svobodnyi Av. , Krasnoyarsk 660041 , Russia.; Institute of Biophysics SB RAS , Federal Research Center 'Krasnoyarsk Science Center SB RAS', 50/50 Akademgorodok , Krasnoyarsk 660036 , Russia., Zhila N; Siberian Federal University , 79 Svobodnyi Av. , Krasnoyarsk 660041 , Russia.; Institute of Biophysics SB RAS , Federal Research Center 'Krasnoyarsk Science Center SB RAS', 50/50 Akademgorodok , Krasnoyarsk 660036 , Russia., Shishatskaya E; Siberian Federal University , 79 Svobodnyi Av. , Krasnoyarsk 660041 , Russia.; Institute of Biophysics SB RAS , Federal Research Center 'Krasnoyarsk Science Center SB RAS', 50/50 Akademgorodok , Krasnoyarsk 660036 , Russia.
Jazyk:	angličtina
Zdroj:	Biomacromolecules [Biomacromolecules] 2019 Sep 09; Vol. 20 (9), pp. 3261-3270. Date of Electronic Publication: 2019 May 23.
DOI:	10.1021/acs.biomac.9b00295
Abstrakt:	The synthesis of polyhydroxyalkanoates (PHAs) was scaled up to pilot production in a 150-L fermenter on sugars (fructose and glucose) and purified and crude glycerol in a culture of the wild-type strain Cupriavidus necator B-10646. Over 60 h of cultivation, a cell concentration of 150-160 g/L was obtained on purified glycerol and glucose; cultivation on fructose and crude glycerol resulted in a cell concentration of 130 ± 10 g/L. Polymer content and yield coefficients for the biomass were similar on all substrates (80-85 wt % and 0.29-0.33 kg biomass/kg carbon substrate, respectively). Copolymers poly(3-hydroxybutyrate- co- 3-hydroxyvalerate) and poly(3-hydroxybutyrate- co -4-hydroxybutyrate) and terpolymers poly(3-hydroxybutyrate- co -3-hydroxyvalerate- co -4-hydroxybutyrate) having a decreased degree of crystallinity (36-46%) were first synthesized in the scaled-up process using C. necator B-10646 cultivated on glycerol. These results will provide the basis for scaling-up PHA synthesis in an organotrophic C. necator B-10646 culture.
Databáze:	MEDLINE
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