Molecular epidemiological analysis of wild animal rabies isolates from India.

Autor: Reddy GBM; Department of Veterinary Pathology, ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru, Karnataka, India., Singh R; Department of Veterinary Pathology, ICAR-Indian Veterinary Research Institute, Bareilly, Uttar Pradesh, India., Singh KP; Department of Veterinary Pathology, ICAR-Indian Veterinary Research Institute, Bareilly, Uttar Pradesh, India., Sharma AK; Department of Veterinary Pathology, ICAR-Indian Veterinary Research Institute, Bareilly, Uttar Pradesh, India., Vineetha S; Department of Veterinary Pathology, ICAR-Indian Veterinary Research Institute, Bareilly, Uttar Pradesh, India., Saminathan M; Department of Veterinary Pathology, ICAR-Indian Veterinary Research Institute, Bareilly, Uttar Pradesh, India., Sajjanar B; Department of Veterinary Pathology, ICAR-Indian Veterinary Research Institute, Bareilly, Uttar Pradesh, India.
Jazyk: angličtina
Zdroj: Veterinary world [Vet World] 2019; Vol. 12 (3), pp. 352-357. Date of Electronic Publication: 2019 Mar 04.
DOI: 10.14202/vetworld.2019.352-357
Abstrakt: Aim: This study was conducted to know the genetic variability of rabies viruses (RVs) from wild animals in India.
Materials and Methods: A total of 20 rabies suspected brain samples of wild animals from different states of India were included in the study. The samples were subjected for direct fluorescent antibody test (dFAT), reverse transcription polymerase chain reaction (RT-PCR), and quantitative reverse transcriptase real-time PCR (RT-qPCR). The phylogenetic analysis of partial nucleoprotein gene sequences was performed.
Results: Of 20 samples, 11, 10, and 12 cases were found positive by dFAT, RT-PCR, and RT-qPCR, respectively. Phylogenetic analysis showed that all Indian wild RVs isolates belonged to classical genotype 1 of Lyssavirus and were closely related to Arctic/Arctic-like single cluster indicating the possibility of a spillover of rabies among different species.
Conclusion: The results indicated the circulation of similar RVs in sylvatic and urban cycles in India. However, understanding the role of wild animals as reservoir host needs to be studied in India.
Databáze: MEDLINE
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