Effects of GABA and Leptin Receptor-Expressing Neurons in the Lateral Hypothalamus on Feeding, Locomotion, and Thermogenesis.

Autor: de Vrind VAJ; Brain Center Rudolf Magnus, Department of Translational Neuroscience, University Medical Center Utrecht and University Utrecht, Utrecht, The Netherlands., Rozeboom A; Brain Center Rudolf Magnus, Department of Translational Neuroscience, University Medical Center Utrecht and University Utrecht, Utrecht, The Netherlands., Wolterink-Donselaar IG; Brain Center Rudolf Magnus, Department of Translational Neuroscience, University Medical Center Utrecht and University Utrecht, Utrecht, The Netherlands., Luijendijk-Berg MCM; Brain Center Rudolf Magnus, Department of Translational Neuroscience, University Medical Center Utrecht and University Utrecht, Utrecht, The Netherlands., Adan RAH; Brain Center Rudolf Magnus, Department of Translational Neuroscience, University Medical Center Utrecht and University Utrecht, Utrecht, The Netherlands.; Institute of Neuroscience and Physiology, The Sahlgrenska Academy at the University of Gothenburg, Gothenburg, Sweden.
Jazyk: angličtina
Zdroj: Obesity (Silver Spring, Md.) [Obesity (Silver Spring)] 2019 Jul; Vol. 27 (7), pp. 1123-1132. Date of Electronic Publication: 2019 May 14.
DOI: 10.1002/oby.22495
Abstrakt: Objective: The lateral hypothalamus (LH) is known for its role in feeding, and it also regulates other aspects of energy homeostasis. How genetically defined LH neuronal subpopulations mediate LH effects on energy homeostasis remains poorly understood. The behavioral effects of chemogenetically activating LH gamma-aminobutyric acid (GABA) and the more selective population of LH GABA neurons that coexpress the leptin receptor (LepR) were compared.
Methods: LepR-cre and VGAT-cre mice were injected with AAV5-hSyn-DIO-hM3DGq-mCherry in the LH. The behavioral effects of LH GABA or LH LepR neuronal activation on feeding, locomotion, thermogenesis, and body weight were assessed.
Results: The activation of LH GABA neurons increased body temperature (P ≤ 0.008) and decreased body weight (P ≤ 0.01) despite decreased locomotor activity (P = 0.03) and transiently increased chow intake (P ≤ 0.009). Also, similar to other studies, this study found that activation of LH GABA neurons induced gnawing on both food and nonfood (P = 0.001) items. Activation of LH LepR neurons decreased body weight (P ≤ 0.01) and chow intake when presented on the cage floor (P ≤ 0.04) but not when presented in the cage top and increased locomotor activity (P = 0.002) and body temperature (P = 0.03).
Conclusions: LH LepR neurons are a subset of LH GABA neurons, and LH LepR activation more specifically regulates energy homeostasis to promote a negative energy balance.
(© 2019 The Authors. Obesity published by Wiley Periodicals, Inc. on behalf of The Obesity Society (TOS).)
Databáze: MEDLINE
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